TY - JOUR
T1 - 12-hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE) stimulates cAMP production in normal human fibroblasts
AU - Ueki, Shoko
AU - Takagi, Junichi
AU - Kobayashi, Yuichi
AU - Sato, Fumie
AU - Saito, Yuji
PY - 1999/1/1
Y1 - 1999/1/1
N2 - We report here that the 12-lipoxygenase metabolite of arachidonic acid, 12-hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE), stimulates cAMP production in human fibroblasts among various cultured cell lines tested. Although 12-HETE seemed to stimulate the phospholipase C (PLC)-protein kinase C (PKC) system, inhibitors against PLC and PKC did not reduce the cAMP production induced by 12-HETE, indicating that the activation of PLC-PKC system is not positively coupled with the stimulation of cAMP production. On the other hand, the cAMP production induced by 12-HETE was dependent on the Ca2+/calmodulin system in the cells. The results suggest that 12-HETE specifically stimulates Ca2+/calmodulin-dependent adenylyl cyclase to increase cAMP level in the fibroblasts.
AB - We report here that the 12-lipoxygenase metabolite of arachidonic acid, 12-hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE), stimulates cAMP production in human fibroblasts among various cultured cell lines tested. Although 12-HETE seemed to stimulate the phospholipase C (PLC)-protein kinase C (PKC) system, inhibitors against PLC and PKC did not reduce the cAMP production induced by 12-HETE, indicating that the activation of PLC-PKC system is not positively coupled with the stimulation of cAMP production. On the other hand, the cAMP production induced by 12-HETE was dependent on the Ca2+/calmodulin system in the cells. The results suggest that 12-HETE specifically stimulates Ca2+/calmodulin-dependent adenylyl cyclase to increase cAMP level in the fibroblasts.
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U2 - 10.1002/(SICI)1097-4652(199901)178:1<63::AID-JCP8>3.0.CO;2-J
DO - 10.1002/(SICI)1097-4652(199901)178:1<63::AID-JCP8>3.0.CO;2-J
M3 - Article
C2 - 9886491
AN - SCOPUS:3743143865
SN - 0021-9541
VL - 178
SP - 63
EP - 68
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 1
ER -