1’prime;-Acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin

Akira Murakami; Shin Ohura; Yoshimasa Nakamura; Koichi Koshimizu; Hajime Ohigashi

doi:10.1159/000227593

1’prime;-Acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin

Akira Murakami, Shin Ohura, Yoshimasa Nakamura, Koichi Koshimizu, Hajime Ohigashi

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

The anti-tumor-promoting activity of 1’-acetoxychavicol acetate (ACA) was examined in a two-stage carcinogenesis experiment in ICR mouse skin using 7,12-dimethylbenz[a]anthracene (0.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice: inhibition ratios 90% (p < 0.001) and 42% (p < 0.005), respectively. ACA even at a dose equimolar to TPA (1.6 nmol) significantly reduced theaverage number of tumors per mouse: inhibitory ratio 44% (p < 0.05). ACA potently inhibited TPA-induced superoxide (O2) generation in differentiated HL-60 cells (IC50 = 4.3μM) and suppressed the lipid hydroperoxide formation by42% (p < 0.001) in the ethyl linoleate autoxidation test.

Original language	English
Pages (from-to)	386-391
Number of pages	6
Journal	Oncology (Switzerland)
Volume	53
Issue number	5
DOIs	https://doi.org/10.1159/000227593
Publication status	Published - Jan 1 1996
Externally published	Yes

Keywords

1’prime;-Acetoxychavicol acetate
Antioxidant
Antitumor promoter
Cancer chemoprevention
HL-60 cells
Languas galanga
Mouse skin
Superoxide anion
Xanthine oxidase

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1159/000227593

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title = "1{\textquoteright}prime;-Acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin",

abstract = "The anti-tumor-promoting activity of 1{\textquoteright}-acetoxychavicol acetate (ACA) was examined in a two-stage carcinogenesis experiment in ICR mouse skin using 7,12-dimethylbenz[a]anthracene (0.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice: inhibition ratios 90% (p < 0.001) and 42% (p < 0.005), respectively. ACA even at a dose equimolar to TPA (1.6 nmol) significantly reduced theaverage number of tumors per mouse: inhibitory ratio 44% (p < 0.05). ACA potently inhibited TPA-induced superoxide (O2) generation in differentiated HL-60 cells (IC50 = 4.3μM) and suppressed the lipid hydroperoxide formation by42% (p < 0.001) in the ethyl linoleate autoxidation test.",

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author = "Akira Murakami and Shin Ohura and Yoshimasa Nakamura and Koichi Koshimizu and Hajime Ohigashi",

year = "1996",

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doi = "10.1159/000227593",

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T1 - 1’prime;-Acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin

AU - Murakami, Akira

AU - Ohura, Shin

AU - Nakamura, Yoshimasa

AU - Koshimizu, Koichi

AU - Ohigashi, Hajime

PY - 1996/1/1

Y1 - 1996/1/1

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AB - The anti-tumor-promoting activity of 1’-acetoxychavicol acetate (ACA) was examined in a two-stage carcinogenesis experiment in ICR mouse skin using 7,12-dimethylbenz[a]anthracene (0.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice: inhibition ratios 90% (p < 0.001) and 42% (p < 0.005), respectively. ACA even at a dose equimolar to TPA (1.6 nmol) significantly reduced theaverage number of tumors per mouse: inhibitory ratio 44% (p < 0.05). ACA potently inhibited TPA-induced superoxide (O2) generation in differentiated HL-60 cells (IC50 = 4.3μM) and suppressed the lipid hydroperoxide formation by42% (p < 0.001) in the ethyl linoleate autoxidation test.

KW - 1’prime;-Acetoxychavicol acetate

KW - Antioxidant

KW - Antitumor promoter

KW - Cancer chemoprevention

KW - HL-60 cells

KW - Languas galanga

KW - Mouse skin

KW - Superoxide anion

KW - Xanthine oxidase

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1’prime;-Acetoxychavicol acetate, a superoxide anion generation inhibitor, potently inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in ICR mouse skin

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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