2,3-Butanedione monoxime suppresses primarily total calcium handling in canine heart

M. Maesako, J. Araki, S. Lee, Y. Doi, T. Imaoka, G. Iribe, S. Mohri, M. Hirakawa, M. Harada, H. Suga

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Whether 2,3-butanedione monoxime (BDM, ≤5mmol/l) suppresses primarily crossbridge cycling or total Ca2+ handling in the blood-perfused whole heart remains controversial. Although BDM seems to suppress primarily total Ca2+ handling in canine hearts, more evidence is lacking. We therefore analyzed the cardiac mechanoenergetics, namely, Emax (contractility), PVA (total mechanical energy), and O2 consumption of canine BDM-treated hearts by our recently developed integrative method to assess myocardial total Ca2+ handling. This method additionally required the internal Ca2+ recirculation fraction. We obtained this from the beat constant of the exponential decay component of the postextrasystolic potentiation. Our analysis indicated significant decreases in both internal Ca2+ recirculation fraction and total Ca2+ handling in the BDM-treated heart, but virtually no change in the reactivity of Emax to total Ca2+ handling. This result corroborates the view that BDM suppresses primarily total Ca2+ handling rather than crossbridge cycling in the canine blood-perfused heart.

Original languageEnglish
Pages (from-to)543-551
Number of pages9
JournalJapanese journal of physiology
Issue number5
Publication statusPublished - 2000
Externally publishedYes


  • Arrhythmia
  • Calcium
  • Contractility
  • Pressure
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Physiology


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