3-dimensional tissue is formed from cancer cells in vitro on gelfoam ®, but not on matrigelTM

Yasunori Tome, Fuminari Uehara, Sumiyuki Mii, Shuya Yano, Lei Zhang, Naotoshi Sugimoto, Hiroki Maehara, Michael Bouvet, Hiroyuki Tsuchiya, Fuminori Kanaya, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam®, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to DNA synthesis and cell growth. Therefore, the flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of αv integrin and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel™, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam®, the cells formed three-dimensional tissue-like structures. The behavior of 143B osteosarcoma cells on Gelfoam ® in culture is remarkably different from those of these cells in monolayer culture or in Matrigel™. Tissue-like structures were observed only in Gelfoam® culture. The data in this report suggest a flexible structural substrate such as Gelfoam® provides a more in vivo-like culture condition than monolayer culture or MatrigelTM and that MatrigelTM does not result in actual three-dimensional culture. J. Cell. Biochem. 115: 1362-1367, 2014.

Original languageEnglish
Pages (from-to)1362-1367
Number of pages6
JournalJournal of Cellular Biochemistry
Issue number8
Publication statusPublished - Aug 2014
Externally publishedYes


  • confocal microscopy
  • culture
  • dimension
  • green fluorescent protein
  • histology
  • matrigel™
  • osteosarcoma
  • real-time imaging

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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