350-kDa royal jelly glycoprotein (Apisin), which stimulates proliferation of human monocytes, bears the β1-3galactosylated N-glycan: Analysis of the N-glycosylation site

Mariko Kimura; Yoshinobu Kimura; Kazunori Tsumura; Kiyoshi Okihara; Hiroyuki Sugimoto; Hideo Yamada; Masami Yonekura

doi:10.1271/bbb.67.2055

350-kDa royal jelly glycoprotein (Apisin), which stimulates proliferation of human monocytes, bears the β1-3galactosylated N-glycan: Analysis of the N-glycosylation site

Mariko Kimura, Yoshinobu Kimura, Kazunori Tsumura, Kiyoshi Okihara, Hiroyuki Sugimoto, Hideo Yamada, Masami Yonekura

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

While doing a structural analysis of minor component N-glycans linked to 350-kDa royal jelly glycoprotein (RJGP), which stimulates the proliferation of human monocytes, we found that a Galβ1-3GlcNAcβ1-4Man unit occurs on the insect glycoprotein. The structure of the fluorescence-labeled N-glycan was analyzed by sugar component analysis, IS-MS, and ¹H-NMR. The structural analysis showed that the 350-kDa RJGP bears Galβ1- 3GlcNAcβ1-4(GlcNAcβ1-2)Manα1-3 (Manα1-3Manα1-6) Manβ1-4GlcNAcβ1-4GlcNAc, suggesting this insect glycoprotein is one of the substrates for both β1-3 galactosyl and β1-4 N-acetyl- glucosamininyl transferases. To our knowledge, this is the first report that succeeded in identifing an insect glycoprotein bearing the β1-3 galactosylated N-glycan.

Original language	English
Pages (from-to)	2055-2058
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	67
Issue number	9
DOIs	https://doi.org/10.1271/bbb.67.2055
Publication status	Published - Sept 2003

Keywords

Apis mellifera
Insect glycoprotein
N-glycosylation
Royal jelly
Site analysis

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.67.2055

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350-kDa royal jelly glycoprotein (Apisin), which stimulates proliferation of human monocytes, bears the β1-3galactosylated N-glycan: Analysis of the N-glycosylation site. / Kimura, Mariko; Kimura, Yoshinobu; Tsumura, Kazunori et al.
In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 9, 09.2003, p. 2055-2058.

Research output: Contribution to journal › Article › peer-review

Kimura, M, Kimura, Y, Tsumura, K, Okihara, K, Sugimoto, H, Yamada, H & Yonekura, M 2003, '350-kDa royal jelly glycoprotein (Apisin), which stimulates proliferation of human monocytes, bears the β1-3galactosylated N-glycan: Analysis of the N-glycosylation site', Bioscience, Biotechnology and Biochemistry, vol. 67, no. 9, pp. 2055-2058. https://doi.org/10.1271/bbb.67.2055

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abstract = "While doing a structural analysis of minor component N-glycans linked to 350-kDa royal jelly glycoprotein (RJGP), which stimulates the proliferation of human monocytes, we found that a Galβ1-3GlcNAcβ1-4Man unit occurs on the insect glycoprotein. The structure of the fluorescence-labeled N-glycan was analyzed by sugar component analysis, IS-MS, and 1H-NMR. The structural analysis showed that the 350-kDa RJGP bears Galβ1- 3GlcNAcβ1-4(GlcNAcβ1-2)Manα1-3 (Manα1-3Manα1-6) Manβ1-4GlcNAcβ1-4GlcNAc, suggesting this insect glycoprotein is one of the substrates for both β1-3 galactosyl and β1-4 N-acetyl- glucosamininyl transferases. To our knowledge, this is the first report that succeeded in identifing an insect glycoprotein bearing the β1-3 galactosylated N-glycan.",

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author = "Mariko Kimura and Yoshinobu Kimura and Kazunori Tsumura and Kiyoshi Okihara and Hiroyuki Sugimoto and Hideo Yamada and Masami Yonekura",

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AU - Yonekura, Masami

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AB - While doing a structural analysis of minor component N-glycans linked to 350-kDa royal jelly glycoprotein (RJGP), which stimulates the proliferation of human monocytes, we found that a Galβ1-3GlcNAcβ1-4Man unit occurs on the insect glycoprotein. The structure of the fluorescence-labeled N-glycan was analyzed by sugar component analysis, IS-MS, and 1H-NMR. The structural analysis showed that the 350-kDa RJGP bears Galβ1- 3GlcNAcβ1-4(GlcNAcβ1-2)Manα1-3 (Manα1-3Manα1-6) Manβ1-4GlcNAcβ1-4GlcNAc, suggesting this insect glycoprotein is one of the substrates for both β1-3 galactosyl and β1-4 N-acetyl- glucosamininyl transferases. To our knowledge, this is the first report that succeeded in identifing an insect glycoprotein bearing the β1-3 galactosylated N-glycan.

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350-kDa royal jelly glycoprotein (Apisin), which stimulates proliferation of human monocytes, bears the β1-3galactosylated N-glycan: Analysis of the N-glycosylation site

Abstract

Keywords

ASJC Scopus subject areas

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