TY - JOUR
T1 - A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans
AU - Kimura, Yoshishige
AU - Corcoran, Ethan E.
AU - Eto, Koh
AU - Gengyo-Ando, Keiko
AU - Muramatsu, Masa Aki
AU - Kobayashi, Ryoji
AU - Freedman, Jonathan H.
AU - Mitani, Shohei
AU - Hagiwara, Masatoshi
AU - Means, Anthony R.
AU - Tokumitsu, Hiroshi
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Calcium (Ca2+) signals regulate a diverse set of cellular responses, from proliferation to muscular contraction and neuro-endocrine secretion. The ubiquitous Ca2+ sensor, calmodulin (CaM), translates changes in local intracellular Ca2+ concentrations into changes in enzyme activities. Among its targets, the Ca2+/CaM-dependent protein kinases I and IV (CaMKs) are capable of transducing intraneuronal signals, and these kinases are implicated in neuronal gene regulation that mediates synaptic plasticity in mammals. Recently, the cyclic AMP response element binding protein (CREB) has been proposed as a target for a CaMK cascade involving not only CaMKI or CaMKIV, but also an upstream kinase kinase that is also CaM regulated (CaMKK). Here, we report that all components of this pathway are coexpressed in head neurons of Caenorhabditis elegans. Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes.
AB - Calcium (Ca2+) signals regulate a diverse set of cellular responses, from proliferation to muscular contraction and neuro-endocrine secretion. The ubiquitous Ca2+ sensor, calmodulin (CaM), translates changes in local intracellular Ca2+ concentrations into changes in enzyme activities. Among its targets, the Ca2+/CaM-dependent protein kinases I and IV (CaMKs) are capable of transducing intraneuronal signals, and these kinases are implicated in neuronal gene regulation that mediates synaptic plasticity in mammals. Recently, the cyclic AMP response element binding protein (CREB) has been proposed as a target for a CaMK cascade involving not only CaMKI or CaMKIV, but also an upstream kinase kinase that is also CaM regulated (CaMKK). Here, we report that all components of this pathway are coexpressed in head neurons of Caenorhabditis elegans. Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes.
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U2 - 10.1093/embo-reports/kvf191
DO - 10.1093/embo-reports/kvf191
M3 - Article
C2 - 12231504
AN - SCOPUS:0036774965
SN - 1469-221X
VL - 3
SP - 962
EP - 966
JO - EMBO Reports
JF - EMBO Reports
IS - 10
ER -