A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis

Lei Zhao; Le Thi Phuong; Mai Thanh Luan; Aprilia Nur Fitrianti; Hidenori Matsui; Hirofumi Nakagami; Yoshiteru Noutoshi; Mikihiro Yamamoto; Yuki Ichinose; Tomonori Shiraishi; Kazuhiro Toyoda

doi:10.1007/s10327-019-00863-9

A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis

Lei Zhao
, Le Thi Phuong
, Mai Thanh Luan
, Aprilia Nur Fitrianti
, Hidenori Matsui
, Hirofumi Nakagami
, Yoshiteru Noutoshi
, Mikihiro Yamamoto
, Yuki Ichinose
, Tomonori Shiraishi
, Kazuhiro Toyoda

School of Agriculture

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

PRX34 mediates the oxidative burst in Arabidopsis. Here we characterized two additional Arabidopsis prx34 null mutants (prx34-2, prx34-3), besides the well-studied prx34-1. Due to a decrease in corresponding peroxidase, the activity that generates reactive oxygen species (ROS) was significantly lower in cell wall extracts of prx34-2 and prx34-3 plants. Consistently, the prx34-2 and prx34-3 exhibited reduced accumulation both of ROS and callose in Flg22-elicitor-treated leaves, leading to enhanced susceptibility to bacterial and fungal pathogens. In contrast, ectopic expression of PRX34 in the wild type caused enhanced resistance. PRX34 is thus a component for disease resistance in Arabidopsis.

Original language	English
Pages (from-to)	405-412
Number of pages	8
Journal	Journal of General Plant Pathology
Volume	85
Issue number	6
DOIs	https://doi.org/10.1007/s10327-019-00863-9
Publication status	Published - Nov 1 2019

Keywords

Apoplastic oxidative burst
Arabidopsis
Cell wall
Class III peroxidase
PRX34
Reactive oxygen species (ROS)

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-019-00863-9

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title = "A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis",

abstract = "PRX34 mediates the oxidative burst in Arabidopsis. Here we characterized two additional Arabidopsis prx34 null mutants (prx34-2, prx34-3), besides the well-studied prx34-1. Due to a decrease in corresponding peroxidase, the activity that generates reactive oxygen species (ROS) was significantly lower in cell wall extracts of prx34-2 and prx34-3 plants. Consistently, the prx34-2 and prx34-3 exhibited reduced accumulation both of ROS and callose in Flg22-elicitor-treated leaves, leading to enhanced susceptibility to bacterial and fungal pathogens. In contrast, ectopic expression of PRX34 in the wild type caused enhanced resistance. PRX34 is thus a component for disease resistance in Arabidopsis.",

keywords = "Apoplastic oxidative burst, Arabidopsis, Cell wall, Class III peroxidase, PRX34, Reactive oxygen species (ROS)",

author = "Lei Zhao and Phuong, \{Le Thi\} and Luan, \{Mai Thanh\} and Fitrianti, \{Aprilia Nur\} and Hidenori Matsui and Hirofumi Nakagami and Yoshiteru Noutoshi and Mikihiro Yamamoto and Yuki Ichinose and Tomonori Shiraishi and Kazuhiro Toyoda",

note = "Publisher Copyright: {\textcopyright} 2019, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.",

year = "2019",

month = nov,

day = "1",

doi = "10.1007/s10327-019-00863-9",

language = "English",

volume = "85",

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TY - JOUR

T1 - A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis

AU - Zhao, Lei

AU - Phuong, Le Thi

AU - Luan, Mai Thanh

AU - Fitrianti, Aprilia Nur

AU - Matsui, Hidenori

AU - Nakagami, Hirofumi

AU - Noutoshi, Yoshiteru

AU - Yamamoto, Mikihiro

AU - Ichinose, Yuki

AU - Shiraishi, Tomonori

AU - Toyoda, Kazuhiro

PY - 2019/11/1

Y1 - 2019/11/1

N2 - PRX34 mediates the oxidative burst in Arabidopsis. Here we characterized two additional Arabidopsis prx34 null mutants (prx34-2, prx34-3), besides the well-studied prx34-1. Due to a decrease in corresponding peroxidase, the activity that generates reactive oxygen species (ROS) was significantly lower in cell wall extracts of prx34-2 and prx34-3 plants. Consistently, the prx34-2 and prx34-3 exhibited reduced accumulation both of ROS and callose in Flg22-elicitor-treated leaves, leading to enhanced susceptibility to bacterial and fungal pathogens. In contrast, ectopic expression of PRX34 in the wild type caused enhanced resistance. PRX34 is thus a component for disease resistance in Arabidopsis.

AB - PRX34 mediates the oxidative burst in Arabidopsis. Here we characterized two additional Arabidopsis prx34 null mutants (prx34-2, prx34-3), besides the well-studied prx34-1. Due to a decrease in corresponding peroxidase, the activity that generates reactive oxygen species (ROS) was significantly lower in cell wall extracts of prx34-2 and prx34-3 plants. Consistently, the prx34-2 and prx34-3 exhibited reduced accumulation both of ROS and callose in Flg22-elicitor-treated leaves, leading to enhanced susceptibility to bacterial and fungal pathogens. In contrast, ectopic expression of PRX34 in the wild type caused enhanced resistance. PRX34 is thus a component for disease resistance in Arabidopsis.

KW - Apoplastic oxidative burst

KW - Arabidopsis

KW - Cell wall

KW - Class III peroxidase

KW - PRX34

KW - Reactive oxygen species (ROS)

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UR - https://www.scopus.com/pages/publications/85066977838#tab=citedBy

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DO - 10.1007/s10327-019-00863-9

M3 - Article

AN - SCOPUS:85066977838

SN - 1345-2630

VL - 85

SP - 405

EP - 412

JO - Journal of General Plant Pathology

JF - Journal of General Plant Pathology

IS - 6

ER -

A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis

Abstract

Keywords

ASJC Scopus subject areas

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