A Clostridium perfringens vector for the selection of promoters

Chieko Matsushita; Osamu Matsushita; Michio Koyama; Akjnobu Okabe

doi:10.1006/plas.1994.1035

A Clostridium perfringens vector for the selection of promoters

Chieko Matsushita, Osamu Matsushita, Michio Koyama, Akjnobu Okabe

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A promoter selection vector for Clostridium perfringens genes was constructed from a C perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from pIP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be a useful reporter system for C. perfringens genes.

Original language	English
Pages (from-to)	317-319
Number of pages	3
Journal	Plasmid
Volume	31
Issue number	3
DOIs	https://doi.org/10.1006/plas.1994.1035
Publication status	Published - May 1994
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology

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10.1006/plas.1994.1035

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AU - Koyama, Michio

AU - Okabe, Akjnobu

PY - 1994/5

Y1 - 1994/5

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AB - A promoter selection vector for Clostridium perfringens genes was constructed from a C perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from pIP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be a useful reporter system for C. perfringens genes.

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A Clostridium perfringens vector for the selection of promoters

Abstract

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