A combination of molecular probe-tandem electrospray ionization mass spectrometry: A technique for tracing structural changes in phospholipid hydroperoxides

Rumiko Shimizu; Ai Nagai; Hiroko Tominaga; Megumi Imura; Arnold N. Onyango; Minoru Izumi; Shuhei Nakajima; Shoichi Tahara; Takao Kaneko; Naomichi Baba

doi:10.1271/bbb.80672

A combination of molecular probe-tandem electrospray ionization mass spectrometry: A technique for tracing structural changes in phospholipid hydroperoxides

Rumiko Shimizu, Ai Nagai, Hiroko Tominaga, Megumi Imura, Arnold N. Onyango, Minoru Izumi, Shuhei Nakajima, Shoichi Tahara, Takao Kaneko, Naomichi Baba

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.

Original language	English
Pages (from-to)	781-784
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	73
Issue number	3
DOIs	https://doi.org/10.1271/bbb.80672
Publication status	Published - 2009

Keywords

ESI MS
Lipid hydroperoxide
Molecular probe
Phosphatidylcholine hydroperoxide
Tandem ESI MS

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.80672

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Shimizu, R., Nagai, A., Tominaga, H., Imura, M., Onyango, A. N., Izumi, M., Nakajima, S., Tahara, S., Kaneko, T., & Baba, N. (2009). A combination of molecular probe-tandem electrospray ionization mass spectrometry: A technique for tracing structural changes in phospholipid hydroperoxides. Bioscience, Biotechnology and Biochemistry, 73(3), 781-784. https://doi.org/10.1271/bbb.80672

Shimizu, R, Nagai, A, Tominaga, H, Imura, M, Onyango, AN, Izumi, M, Nakajima, S, Tahara, S, Kaneko, T & Baba, N 2009, 'A combination of molecular probe-tandem electrospray ionization mass spectrometry: A technique for tracing structural changes in phospholipid hydroperoxides', Bioscience, Biotechnology and Biochemistry, vol. 73, no. 3, pp. 781-784. https://doi.org/10.1271/bbb.80672

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abstract = "An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.",

keywords = "ESI MS, Lipid hydroperoxide, Molecular probe, Phosphatidylcholine hydroperoxide, Tandem ESI MS",

author = "Rumiko Shimizu and Ai Nagai and Hiroko Tominaga and Megumi Imura and Onyango, {Arnold N.} and Minoru Izumi and Shuhei Nakajima and Shoichi Tahara and Takao Kaneko and Naomichi Baba",

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AU - Shimizu, Rumiko

AU - Nagai, Ai

AU - Tominaga, Hiroko

AU - Imura, Megumi

AU - Onyango, Arnold N.

AU - Izumi, Minoru

AU - Nakajima, Shuhei

AU - Tahara, Shoichi

AU - Kaneko, Takao

AU - Baba, Naomichi

PY - 2009

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AB - An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.

KW - ESI MS

KW - Lipid hydroperoxide

KW - Molecular probe

KW - Phosphatidylcholine hydroperoxide

KW - Tandem ESI MS

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A combination of molecular probe-tandem electrospray ionization mass spectrometry: A technique for tracing structural changes in phospholipid hydroperoxides

Abstract

Keywords

ASJC Scopus subject areas

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