A coplanar PCB induces a selenium binding protein as a major cytosolic protein in rat liver

We obtained evidence that a toxic coplanar polychlorinated biphenyl (PCB) induces a counterpart,of murine 56 kDa selenium binding protein in rat liver cytosol. A 54 kDa protein in the liver cytosol was significantly induced by 3,3',4,4',5-pentachlorobiphenyl and proved to be a major cytosolic protein in the rat liver. The protein exhibited pi of 6.8 on two-dimensional gel electrophoresis. The amino acid sequence of peptide fragments from the protein digested in situ, was highly similar to a 56 kDa selenium binding protein and similar to an acetaminophen binding protein in mice.