A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease

Satsuki Sato; Yohei Kamata; Takaomi Kessoku; Tomoko Shimizu; Takashi Kobayashi; Takeo Kurihashi; Shogo Takashiba; Kazu Hatanaka; Nobushiro Hamada; Toshiro Kodama; Takuma Higurashi; Masataka Taguri; Masato Yoneda; Haruki Usuda; Koichiro Wada; Atsushi Nakajima; Toshiya Morozumi; Masato Minabe

doi:10.1038/s41598-022-17917-2

A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease

Satsuki Sato, Yohei Kamata, Takaomi Kessoku, Tomoko Shimizu, Takashi Kobayashi, Takeo Kurihashi, Shogo Takashiba, Kazu Hatanaka, Nobushiro Hamada, Toshiro Kodama, Takuma Higurashi, Masataka Taguri, Masato Yoneda, Haruki Usuda, Koichiro Wada, Atsushi Nakajima, Toshiya Morozumi, Masato Minabe

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The risk factors for non-alcoholic fatty liver disease (NAFLD) progression are not completely known. Porphyromonasgingivalis infection is a risk factor for systemic diseases. We investigated the association of P.gingivalis infection with the risk of non-alcoholic steatohepatitis progression. Here, hematological tests, periodontal examination, and saliva collection were performed for 164 patients with NAFLD. P.gingivalis was identified in saliva using polymerase chain reaction. Hepatic steatosis and stiffness were evaluated using vibration-controlled transient elastography (VCTE) and magnetic resonance imaging. In patients with NAFLD, P.gingivalis positivity (P.gingivalis ratio ≥ 0.01%) in saliva correlated with liver stiffness determined using magnetic resonance elastography (MRE; p < 0.0001). A P.gingivalis ratio of 0.01% corresponds to 100,000 cells/mL and indicates the proportion of P.gingivalis in the total number of bacteria in the oral cavity. Patients with NAFLD and advanced fibrosis on MRE showed significantly elevated endotoxin activity; those who had > 10 periodontal pockets with depths ≥ 4 mm had significantly increased hepatic stiffness on both VCTE and MRE.

Original language	English
Article number	13621
Journal	Scientific reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-17917-2
Publication status	Published - Dec 2022
Externally published	Yes

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Sato, S., Kamata, Y., Kessoku, T., Shimizu, T., Kobayashi, T., Kurihashi, T., Takashiba, S., Hatanaka, K., Hamada, N., Kodama, T., Higurashi, T., Taguri, M., Yoneda, M., Usuda, H., Wada, K., Nakajima, A., Morozumi, T., & Minabe, M. (2022). A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease. Scientific reports, 12(1), [13621]. https://doi.org/10.1038/s41598-022-17917-2

Sato, S, Kamata, Y, Kessoku, T, Shimizu, T, Kobayashi, T, Kurihashi, T, Takashiba, S, Hatanaka, K, Hamada, N, Kodama, T, Higurashi, T, Taguri, M, Yoneda, M, Usuda, H, Wada, K, Nakajima, A, Morozumi, T & Minabe, M 2022, 'A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease', Scientific reports, vol. 12, no. 1, 13621. https://doi.org/10.1038/s41598-022-17917-2

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title = "A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease",

abstract = "The risk factors for non-alcoholic fatty liver disease (NAFLD) progression are not completely known. Porphyromonasgingivalis infection is a risk factor for systemic diseases. We investigated the association of P.gingivalis infection with the risk of non-alcoholic steatohepatitis progression. Here, hematological tests, periodontal examination, and saliva collection were performed for 164 patients with NAFLD. P.gingivalis was identified in saliva using polymerase chain reaction. Hepatic steatosis and stiffness were evaluated using vibration-controlled transient elastography (VCTE) and magnetic resonance imaging. In patients with NAFLD, P.gingivalis positivity (P.gingivalis ratio ≥ 0.01%) in saliva correlated with liver stiffness determined using magnetic resonance elastography (MRE; p < 0.0001). A P.gingivalis ratio of 0.01% corresponds to 100,000 cells/mL and indicates the proportion of P.gingivalis in the total number of bacteria in the oral cavity. Patients with NAFLD and advanced fibrosis on MRE showed significantly elevated endotoxin activity; those who had > 10 periodontal pockets with depths ≥ 4 mm had significantly increased hepatic stiffness on both VCTE and MRE.",

author = "Satsuki Sato and Yohei Kamata and Takaomi Kessoku and Tomoko Shimizu and Takashi Kobayashi and Takeo Kurihashi and Shogo Takashiba and Kazu Hatanaka and Nobushiro Hamada and Toshiro Kodama and Takuma Higurashi and Masataka Taguri and Masato Yoneda and Haruki Usuda and Koichiro Wada and Atsushi Nakajima and Toshiya Morozumi and Masato Minabe",

note = "Funding Information: This investigator-initiated cross-sectional study was conducted by the Kanagawa Dental University—as the sponsor—and funded by the Ministry of Health, Labour, and Welfare, Japan (Grant number: 16K11872). The funders of this trial played no role in the design of this study; collection, analysis, and interpretation of data; or writing of the manuscript. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1038/s41598-022-17917-2",

language = "English",

volume = "12",

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T1 - A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease

AU - Sato, Satsuki

AU - Kamata, Yohei

AU - Kessoku, Takaomi

AU - Shimizu, Tomoko

AU - Kobayashi, Takashi

AU - Kurihashi, Takeo

AU - Takashiba, Shogo

AU - Hatanaka, Kazu

AU - Hamada, Nobushiro

AU - Kodama, Toshiro

AU - Higurashi, Takuma

AU - Taguri, Masataka

AU - Yoneda, Masato

AU - Usuda, Haruki

AU - Wada, Koichiro

AU - Nakajima, Atsushi

AU - Morozumi, Toshiya

AU - Minabe, Masato

N1 - Funding Information: This investigator-initiated cross-sectional study was conducted by the Kanagawa Dental University—as the sponsor—and funded by the Ministry of Health, Labour, and Welfare, Japan (Grant number: 16K11872). The funders of this trial played no role in the design of this study; collection, analysis, and interpretation of data; or writing of the manuscript. Publisher Copyright: © 2022, The Author(s).

PY - 2022/12

Y1 - 2022/12

N2 - The risk factors for non-alcoholic fatty liver disease (NAFLD) progression are not completely known. Porphyromonasgingivalis infection is a risk factor for systemic diseases. We investigated the association of P.gingivalis infection with the risk of non-alcoholic steatohepatitis progression. Here, hematological tests, periodontal examination, and saliva collection were performed for 164 patients with NAFLD. P.gingivalis was identified in saliva using polymerase chain reaction. Hepatic steatosis and stiffness were evaluated using vibration-controlled transient elastography (VCTE) and magnetic resonance imaging. In patients with NAFLD, P.gingivalis positivity (P.gingivalis ratio ≥ 0.01%) in saliva correlated with liver stiffness determined using magnetic resonance elastography (MRE; p < 0.0001). A P.gingivalis ratio of 0.01% corresponds to 100,000 cells/mL and indicates the proportion of P.gingivalis in the total number of bacteria in the oral cavity. Patients with NAFLD and advanced fibrosis on MRE showed significantly elevated endotoxin activity; those who had > 10 periodontal pockets with depths ≥ 4 mm had significantly increased hepatic stiffness on both VCTE and MRE.

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JO - Scientific Reports

JF - Scientific Reports

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M1 - 13621

ER -

A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease

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