@article{aa3edafe9aa346f3becd7c488ed637f5,
title = "A gold nano-electrode for single ion channel recordings",
abstract = "The artificial bilayer single channel recording technique is commonly used to observe the detailed physiological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we developed a novel artificial bilayer single channel recording technique in which solubilized ion channel proteins immobilized on a gold nano-electrode are directly incorporated into a lipid bilayer at the same time as the bilayer is formed at the tip of it on coming in contact with an aqueous-oil interface. Using this technique, we measured the single channel currents of several types of channels including KcsA, MthK, hBK and P2X4. This technique requires only one action to simultaneously form the bilayers and reconstitute the channels into the membranes. This simplicity greatly increases the measurement efficiency and allows the technique to potentially be combined with high-throughput screening devices.",
author = "Daichi Okuno and Minako Hirano and Hiroaki Yokota and Junya Ichinose and Takamitsu Kira and Taiki Hijiya and Chihiro Uozumi and Masahiro Yamakami and Toru Ide",
note = "Funding Information: We thank Dr Tai Kubo (Advanced Industrial Science and Technology, Tsukuba, Japan) for providing us the plasmids pQE-30/KcsA and pSD64/P2X4, Dr Youxing Jiang (University of Texas Southwestern Medical Center, Dallas, TX) for providing us the plasmid with MthK, and Dr Alice Y. Ting (Massachusetts Institute of Technology, Cambridge, MA) for providing pET21a-BirA, which is publicly available through Addgene. The authors thank Dr Peter Karagiannis for carefully revising the manuscript. This work was supported by grants from the Research Foundation for Opto-Science and Technology, the NOVARTIS Foundation (Japan) for the Promotion of Science, the Kurata Memorial Hitachi Science and Technology Foundation, the JSPS KAKENHI grant numbers 22370059 and 24657110, Grants-in-aid for Scientific Research of Foundation Advanced Technology Institute (ATI) and the Science Research Promotion Fund of The Promotion and Mutual Aid Corporation for Private Schools of Japan. Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",
year = "2018",
month = feb,
day = "28",
doi = "10.1039/c7nr08098k",
language = "English",
volume = "10",
pages = "4036--4040",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "Royal Society of Chemistry",
number = "8",
}