A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis

Kosei Hasegawa; Toshiro Ono; Hirokazu Matsushita; Michihide Shimono; Yuji Noguchi; Yasushi Mizutani; Junichi Kodama; Takafumi Kudo; Eiichi Nakayama

doi:10.1002/ijc.11565

A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis

Kosei Hasegawa, Toshiro Ono, Hirokazu Matsushita, Michihide Shimono, Yuji Noguchi, Yasushi Mizutani, Junichi Kodama, Takafumi Kudo, Eiichi Nakayama

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A-kinase anchoring protein 3 (AKAP3) is a sperm protein and its expression appears to be restricted to the testis in normal adult tissues. We investigated AKAP3 mRNA expression in 20 normal ovaries and 54 ovarian cancers of different histological types, grades and stages by reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were analyzed by conventional agarose gel electrophoresis and capillary electrophoresis on a microtip device to determine the expression semiquantitatively. Little or no expression was observed in the 20 normal ovarian specimens. High AKAP3 mRNA expression was observed in 15 ovarian cancer specimens (28 %). The expression was correlated with the histological grade and clinical stage. AKAP3 mRNA was observed at a significantly higher frequency in poorly differentiated (p = 0.009) and advanced stage (III and IV, p = 0.014) tumors. No correlation was found between AKAP3 mRNA expression and other variables. In Cox multivariate analysis, AKAP3 mRNA expression was found to be a significant predictor of both overall and progression-free survival in patients with poorly differentiated tumors.

Original language	English
Pages (from-to)	86-90
Number of pages	5
Journal	International Journal of Cancer
Volume	108
Issue number	1
DOIs	https://doi.org/10.1002/ijc.11565
Publication status	Published - Jan 1 2004
Externally published	Yes

Keywords

AKAP
Cancer antigen
Ovarian cancer
Prognosis

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/ijc.11565

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title = "A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis",

abstract = "A-kinase anchoring protein 3 (AKAP3) is a sperm protein and its expression appears to be restricted to the testis in normal adult tissues. We investigated AKAP3 mRNA expression in 20 normal ovaries and 54 ovarian cancers of different histological types, grades and stages by reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were analyzed by conventional agarose gel electrophoresis and capillary electrophoresis on a microtip device to determine the expression semiquantitatively. Little or no expression was observed in the 20 normal ovarian specimens. High AKAP3 mRNA expression was observed in 15 ovarian cancer specimens (28 %). The expression was correlated with the histological grade and clinical stage. AKAP3 mRNA was observed at a significantly higher frequency in poorly differentiated (p = 0.009) and advanced stage (III and IV, p = 0.014) tumors. No correlation was found between AKAP3 mRNA expression and other variables. In Cox multivariate analysis, AKAP3 mRNA expression was found to be a significant predictor of both overall and progression-free survival in patients with poorly differentiated tumors.",

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AU - Hasegawa, Kosei

AU - Ono, Toshiro

AU - Matsushita, Hirokazu

AU - Shimono, Michihide

AU - Noguchi, Yuji

AU - Mizutani, Yasushi

AU - Kodama, Junichi

AU - Kudo, Takafumi

AU - Nakayama, Eiichi

PY - 2004/1/1

Y1 - 2004/1/1

N2 - A-kinase anchoring protein 3 (AKAP3) is a sperm protein and its expression appears to be restricted to the testis in normal adult tissues. We investigated AKAP3 mRNA expression in 20 normal ovaries and 54 ovarian cancers of different histological types, grades and stages by reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were analyzed by conventional agarose gel electrophoresis and capillary electrophoresis on a microtip device to determine the expression semiquantitatively. Little or no expression was observed in the 20 normal ovarian specimens. High AKAP3 mRNA expression was observed in 15 ovarian cancer specimens (28 %). The expression was correlated with the histological grade and clinical stage. AKAP3 mRNA was observed at a significantly higher frequency in poorly differentiated (p = 0.009) and advanced stage (III and IV, p = 0.014) tumors. No correlation was found between AKAP3 mRNA expression and other variables. In Cox multivariate analysis, AKAP3 mRNA expression was found to be a significant predictor of both overall and progression-free survival in patients with poorly differentiated tumors.

AB - A-kinase anchoring protein 3 (AKAP3) is a sperm protein and its expression appears to be restricted to the testis in normal adult tissues. We investigated AKAP3 mRNA expression in 20 normal ovaries and 54 ovarian cancers of different histological types, grades and stages by reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were analyzed by conventional agarose gel electrophoresis and capillary electrophoresis on a microtip device to determine the expression semiquantitatively. Little or no expression was observed in the 20 normal ovarian specimens. High AKAP3 mRNA expression was observed in 15 ovarian cancer specimens (28 %). The expression was correlated with the histological grade and clinical stage. AKAP3 mRNA was observed at a significantly higher frequency in poorly differentiated (p = 0.009) and advanced stage (III and IV, p = 0.014) tumors. No correlation was found between AKAP3 mRNA expression and other variables. In Cox multivariate analysis, AKAP3 mRNA expression was found to be a significant predictor of both overall and progression-free survival in patients with poorly differentiated tumors.

KW - AKAP

KW - Cancer antigen

KW - Ovarian cancer

KW - Prognosis

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A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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