A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Yoshiyuki Hakata; Hiroyuki Michiue; Takashi Ohtsuki; Masaaki Miyazawa; Mizuki Kitamatsu

doi:10.1016/j.bmcl.2019.02.004

A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Yoshiyuki Hakata
, Hiroyuki Michiue
, Takashi Ohtsuki
, Masaaki Miyazawa
, Mizuki Kitamatsu

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

Original language	English
Pages (from-to)	878-881
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	29
Issue number	7
DOIs	https://doi.org/10.1016/j.bmcl.2019.02.004
Publication status	Published - Apr 1 2019

Keywords

Cell-penetrating peptide
Drug delivery system
Leucine zipper
Nanog
Peptide

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2019.02.004

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title = "A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus",

abstract = "We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.",

keywords = "Cell-penetrating peptide, Drug delivery system, Leucine zipper, Nanog, Peptide",

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T1 - A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

AU - Hakata, Yoshiyuki

AU - Michiue, Hiroyuki

AU - Ohtsuki, Takashi

AU - Miyazawa, Masaaki

AU - Kitamatsu, Mizuki

PY - 2019/4/1

Y1 - 2019/4/1

N2 - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

AB - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

KW - Cell-penetrating peptide

KW - Drug delivery system

KW - Leucine zipper

KW - Nanog

KW - Peptide

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M3 - Article

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AN - SCOPUS:85060977862

SN - 0960-894X

VL - 29

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 7

ER -

A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Abstract

Keywords

ASJC Scopus subject areas

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