A lipid bilayer formed on a hydrogel bead for single ion channel recordings

Minako Hirano; Daiki Yamamoto; Mami Asakura; Tohru Hayakawa; Shintaro Mise; Akinobu Matsumoto; Toru Ide

doi:10.3390/mi11121070

A lipid bilayer formed on a hydrogel bead for single ion channel recordings

Minako Hirano, Daiki Yamamoto, Mami Asakura, Tohru Hayakawa, Shintaro Mise, Akinobu Matsumoto, Toru Ide

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage-and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

Original language	English
Article number	1070
Pages (from-to)	1-15
Number of pages	15
Journal	Micromachines
Volume	11
Issue number	12
DOIs	https://doi.org/10.3390/mi11121070
Publication status	Published - Dec 2020

Keywords

Ion channel
Lipid bilayer
Single-channel recording

ASJC Scopus subject areas

Control and Systems Engineering
Mechanical Engineering
Electrical and Electronic Engineering

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10.3390/mi11121070

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title = "A lipid bilayer formed on a hydrogel bead for single ion channel recordings",

abstract = "Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage-and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.",

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note = "Funding Information: Funding: This research was funded by JSPS KAKENHI, grant numbers 15K13725 and 18K06157, MEXT Program for Building Regional Innovation Ecosystems. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/mi11121070",

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AU - Hirano, Minako

AU - Yamamoto, Daiki

AU - Asakura, Mami

AU - Hayakawa, Tohru

AU - Mise, Shintaro

AU - Matsumoto, Akinobu

AU - Ide, Toru

N1 - Funding Information: Funding: This research was funded by JSPS KAKENHI, grant numbers 15K13725 and 18K06157, MEXT Program for Building Regional Innovation Ecosystems. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/12

Y1 - 2020/12

N2 - Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage-and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

AB - Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage-and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

KW - Ion channel

KW - Lipid bilayer

KW - Single-channel recording

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A lipid bilayer formed on a hydrogel bead for single ion channel recordings

Abstract

Keywords

ASJC Scopus subject areas

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