TY - JOUR
T1 - A morphological study on glutamate-induced swelling of cultured astrocytes
T2 - Involvement of calcium and chloride ion mechanisms
AU - Koyama, Yutaka
AU - Sugimoto, Tomosada
AU - Shigenaga, Yoshio
AU - Baba, Akemichi
AU - Iwata, Heitaroh
PY - 1991/4/1
Y1 - 1991/4/1
N2 - Morphological changes in cultured astrocytes exposed to l-glutamate (Glu) were examined light and electron microscopically. The treatment with 0.1 mM Glu for 60 min caused marked swelling of the cells, which was characterized by reduction in staining of cytoplasm with Toluidine blue, disappearance of the cytoplasmic granular ground substances, swollen mitochondrion and nucleus, and dispersed chromatin. The above changes were prevented by the removal of Na+, Ca2+ or Cl- from the incubation medium for Glu treatment. However, the Glu treatment in a Cl--free medium caused conspicuous aggregation of 10 nm filaments.
AB - Morphological changes in cultured astrocytes exposed to l-glutamate (Glu) were examined light and electron microscopically. The treatment with 0.1 mM Glu for 60 min caused marked swelling of the cells, which was characterized by reduction in staining of cytoplasm with Toluidine blue, disappearance of the cytoplasmic granular ground substances, swollen mitochondrion and nucleus, and dispersed chromatin. The above changes were prevented by the removal of Na+, Ca2+ or Cl- from the incubation medium for Glu treatment. However, the Glu treatment in a Cl--free medium caused conspicuous aggregation of 10 nm filaments.
KW - Astrocyte
KW - Ca
KW - Swelling
KW - Ultrastructure
KW - l-Glutamate
UR - http://www.scopus.com/inward/record.url?scp=0025796659&partnerID=8YFLogxK
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U2 - 10.1016/0304-3940(91)90102-Y
DO - 10.1016/0304-3940(91)90102-Y
M3 - Article
C2 - 1676835
AN - SCOPUS:0025796659
SN - 0304-3940
VL - 124
SP - 235
EP - 238
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -