Abstract
We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male : female ratio as low as 1:10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described.
| Original language | English |
|---|---|
| Pages (from-to) | 113-122 |
| Number of pages | 10 |
| Journal | Acta medica Okayama |
| Volume | 65 |
| Issue number | 2 |
| Publication status | Published - 2011 |
Keywords
- Forensic casework
- Multiplex PCR
- SRY (sex-determining region Y)
- Sex determination
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)