TY - JOUR
T1 - A new resistance gene in combination with Rmg8 confers strong resistance against triticum isolates of Pyricularia oryzae in a common wheat landrace
AU - Wang, Shizhen
AU - Asuke, Soichiro
AU - Vy, Trinh Thi Phuong
AU - Inoue, Yoshihiro
AU - Chuma, Izumi
AU - Win, Joe
AU - Kato, Kenji
AU - Tosa, Yukio
N1 - Funding Information:
Funding: This work was supported by the Japan Society for the Promotion of Science grant 39001248. J. Win was funded by the Gatsby Charitable foundation.
Publisher Copyright:
© 2018 The American Phytopathological Society.
PY - 2018/11
Y1 - 2018/11
N2 - The wheat blast fungus (Triticum pathotype of Pyricularia oryzae) first arose in Brazil in 1985 and has recently spread to Asia. Resistance genes against this new pathogen are very rare in common wheat populations. We screened 520 local landraces of common wheat collected worldwide with Br48, a Triticum isolate collected in Brazil, and found a highly resistant, unique accession, GR119. When F2 seedlings derived from a cross between GR119 and Chinese Spring (CS, susceptible control) were inoculated with Br48, resistant and susceptible seedlings segregated in a 15:1 ratio, suggesting that GR119 carries two resistance genes. When the F2 seedlings were inoculated with Br48DA8 carrying a disrupted allele of AVR-Rmg8 (an avirulence gene corresponding to a previously reported resistance gene, Rmg8), however, the segregation fitted a 3:1 ratio. These results suggest that one of the two genes in GR119 was Rmg8. The other, new gene was tentatively designated as RmgGR119. GR119 was highly resistant to all Triticum isolates tested. Spikes of GR119 were highly resistant to Br48, moderately resistant to Br48DA8 and a hybrid culture carrying avr-Rmg8 (nonfunctional allele), and highly resistant to its transformant carrying AVR-Rmg8. The strong resistance of GR119 was attributed to the combined effects of Rmg8 and RmgGR119.
AB - The wheat blast fungus (Triticum pathotype of Pyricularia oryzae) first arose in Brazil in 1985 and has recently spread to Asia. Resistance genes against this new pathogen are very rare in common wheat populations. We screened 520 local landraces of common wheat collected worldwide with Br48, a Triticum isolate collected in Brazil, and found a highly resistant, unique accession, GR119. When F2 seedlings derived from a cross between GR119 and Chinese Spring (CS, susceptible control) were inoculated with Br48, resistant and susceptible seedlings segregated in a 15:1 ratio, suggesting that GR119 carries two resistance genes. When the F2 seedlings were inoculated with Br48DA8 carrying a disrupted allele of AVR-Rmg8 (an avirulence gene corresponding to a previously reported resistance gene, Rmg8), however, the segregation fitted a 3:1 ratio. These results suggest that one of the two genes in GR119 was Rmg8. The other, new gene was tentatively designated as RmgGR119. GR119 was highly resistant to all Triticum isolates tested. Spikes of GR119 were highly resistant to Br48, moderately resistant to Br48DA8 and a hybrid culture carrying avr-Rmg8 (nonfunctional allele), and highly resistant to its transformant carrying AVR-Rmg8. The strong resistance of GR119 was attributed to the combined effects of Rmg8 and RmgGR119.
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U2 - 10.1094/PHYTO-12-17-0400-R
DO - 10.1094/PHYTO-12-17-0400-R
M3 - Article
C2 - 29767554
AN - SCOPUS:85055214896
SN - 0031-949X
VL - 108
SP - 1299
EP - 1306
JO - Phytopathology
JF - Phytopathology
IS - 11
ER -