TY - JOUR
T1 - A novel deltapartitivirus from red clover
AU - Telengech, Paul
AU - Shahi, Sabitree
AU - Kondo, Hideki
AU - Suzuki, Nobuhiro
N1 - Funding Information:
SS is a JSPS fellow. The authors are grateful to Tokachi Station, National Livestock Breeding Center (NLBC) for the generous gift of the three red clover cultivars (Hokuseki, Natsuyu, and Taisetsu). PT is thankful to the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan for a MEXT scholarship.
Funding Information:
SS is a JSPS fellow. The authors are grateful to Tokachi Station, National Livestock Breeding Center (NLBC) for the generous gift of the three red clover cultivars (Hokuseki, Natsuyu, and Taisetsu). PT is thankful to the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan for a MEXT scholarship.
Funding Information:
This study was supported in part by Yomogi Inc., the Ohara Foundation for Agriculture Research (to NS), and Grants-in-Aid for Scientific Research on Innovative Areas from the Japanese Ministry of Education, Culture, Sports, Science and Technology (KAKENHI 21H05035, 21K18222, 20H02987, 16H06436, 16H06429 and 16K21723 to NS and HK). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2022/4
Y1 - 2022/4
N2 - The family Partitiviridae has five genera, among which is the genus Deltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed “red clover cryptic virus 3” (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genus Deltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.
AB - The family Partitiviridae has five genera, among which is the genus Deltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed “red clover cryptic virus 3” (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genus Deltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.
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U2 - 10.1007/s00705-022-05372-3
DO - 10.1007/s00705-022-05372-3
M3 - Article
C2 - 35246731
AN - SCOPUS:85126114192
SN - 0304-8608
VL - 167
SP - 1201
EP - 1204
JO - Archives of Virology
JF - Archives of Virology
IS - 4
ER -