TY - JOUR
T1 - A novel real-time DNA detection system for loop-mediated isothermal amplification method
AU - Kakugawa, Koji
AU - Yamada, Kenji
AU - Maeda, Hiroshi
AU - Takashiba, Shougo
PY - 2010
Y1 - 2010
N2 - We developed a novel real-time DNA detection system for loop-mediated isothermal amplification (LAMP) method. Our prototype was composed of a thermostatic chamber, a hole slide glass, LED and a web camera. The reaction mixture was injected into the slide glass hole and the LAMP reaction was carried out at 63°C for 2 hours. To observe the DNA amplification, we monitored the fluorescence intensity of SYBR Green I that was excited by the blue LED. The captured BMP images were analyzed by NIH Image J software. The DNA amplification and amplification monitoring experiment was successful. Furthermore, quantitative accuracy was evaluated based on real-time PCR. The reaction time correlates well with the DNA concentration. These results indicate the successful development of a novel real-time DNA detection system for LAMP method.
AB - We developed a novel real-time DNA detection system for loop-mediated isothermal amplification (LAMP) method. Our prototype was composed of a thermostatic chamber, a hole slide glass, LED and a web camera. The reaction mixture was injected into the slide glass hole and the LAMP reaction was carried out at 63°C for 2 hours. To observe the DNA amplification, we monitored the fluorescence intensity of SYBR Green I that was excited by the blue LED. The captured BMP images were analyzed by NIH Image J software. The DNA amplification and amplification monitoring experiment was successful. Furthermore, quantitative accuracy was evaluated based on real-time PCR. The reaction time correlates well with the DNA concentration. These results indicate the successful development of a novel real-time DNA detection system for LAMP method.
KW - Aspiration pneumonia
KW - DNA
KW - Lamp method
KW - Oral care
KW - Periodontal pathogen
UR - http://www.scopus.com/inward/record.url?scp=77953529050&partnerID=8YFLogxK
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U2 - 10.1541/ieejeiss.130.807
DO - 10.1541/ieejeiss.130.807
M3 - Article
AN - SCOPUS:77953529050
SN - 0385-4221
VL - 130
SP - 807-812+10
JO - IEEJ Transactions on Electronics, Information and Systems
JF - IEEJ Transactions on Electronics, Information and Systems
IS - 5
ER -