A novel site-specific recombination system derived from bacteriophage φ{symbol}MR11

Mohammad Rashel, Jumpei Uchiyama, Takako Ujihara, Iyo Takemura, Hiroshi Hoshiba, Shigenobu Matsuzaki

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


We report identification of a novel site-specific DNA recombination system that functions in both in vivo and in vitro, derived from lysogenic Staphylococcus aureus phage φ{symbol}MR11. In silico analysis of the φ{symbol}MR11 genome indicated orf1 as a putative integrase gene. Phage and bacterial attachment sites (attP and attB, respectively) and attachment junctions were determined and their nucleotide sequences decoded. Sequences of attP and attB were mostly different to each other except for a two bp common core that was the crossover point. We found several inverted repeats adjacent to the core sequence of attP as potential protein binding sites. The precise and efficient integration properties of φ{symbol}MR11 integrase were shown on attP and attB in Escherichia coli and the minimum size of attP was found to be 34 bp. In in vitro assays using crude or purified integrase, only buffer and substrate DNAs were required for the recombination reaction, indicating that other bacterially encoded factors are not essential for activity.

Original languageEnglish
Pages (from-to)192-198
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Apr 4 2008
Externally publishedYes


  • Bacteriophage φ{symbol}MR11
  • Integrase
  • S. aureus
  • Serine recombinase
  • Site-specific recombination

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'A novel site-specific recombination system derived from bacteriophage φ{symbol}MR11'. Together they form a unique fingerprint.

Cite this