TY - JOUR
T1 - A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum
AU - Wang, Shuangchao
AU - Kondo, Hideki
AU - Liu, Liang
AU - Guo, Lihua
AU - Qiu, Dewen
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 31171818 ). We thank Nobuhiro Suzuki for fruitful discussion and critical reading of the manuscript. We acknowledge generous gifts of fungal strains from Linghuo Jiang.
PY - 2013/6
Y1 - 2013/6
N2 - •We isolated a novel mycovirus from the plant pathogenic fungus F. graminearum.•We sequenced the full-length of the virus with a genome of 13,023 nucleotides.•Phylogenetic analysis suggested FgHV1 was closely related to CHV1 and CHV2.•FgHV1 infection resulted in no dramatic phenotypic alteration to the host fungus. A double-stranded (ds) RNA element, sized at approximately 13. kb pairs, was purified from a field isolate, HN10, of Fusarium graminearum. The coding strand of the dsRNA was 13,023 nucleotides (nt) long (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORF A and ORF B). The 5' proximal ORF A of 531. nt encoded a protein of 176 amino acids (aa), and a BLAST search showed it to be similar to the putative papain-like protease domains encoded by Valsa ceratosperma hypovirus 1 (35% identity) and Cryphonectria hypovirus 4 (CHV4) (31% identity). The 3' proximal ORF B of 11,118. nt encoded a large polyprotein with three conserved domains, including papain-like protease, RNA-dependent RNA polymerase and RNA helicase domains. The polyprotein shared significant aa identities with CHV1 (32%) and CHV2 (32%). Both the genome organization and phylogenetic analysis suggested that the characterized RNA represented a novel hypovirus, designated "Fusarium graminearum hypovirus 1 (FgHV1)", which was closely related to CHV1 and CHV2 in the Hypoviridae family. Elimination of the virus resulted in no dramatic phenotypic alteration of the fungus.
AB - •We isolated a novel mycovirus from the plant pathogenic fungus F. graminearum.•We sequenced the full-length of the virus with a genome of 13,023 nucleotides.•Phylogenetic analysis suggested FgHV1 was closely related to CHV1 and CHV2.•FgHV1 infection resulted in no dramatic phenotypic alteration to the host fungus. A double-stranded (ds) RNA element, sized at approximately 13. kb pairs, was purified from a field isolate, HN10, of Fusarium graminearum. The coding strand of the dsRNA was 13,023 nucleotides (nt) long (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORF A and ORF B). The 5' proximal ORF A of 531. nt encoded a protein of 176 amino acids (aa), and a BLAST search showed it to be similar to the putative papain-like protease domains encoded by Valsa ceratosperma hypovirus 1 (35% identity) and Cryphonectria hypovirus 4 (CHV4) (31% identity). The 3' proximal ORF B of 11,118. nt encoded a large polyprotein with three conserved domains, including papain-like protease, RNA-dependent RNA polymerase and RNA helicase domains. The polyprotein shared significant aa identities with CHV1 (32%) and CHV2 (32%). Both the genome organization and phylogenetic analysis suggested that the characterized RNA represented a novel hypovirus, designated "Fusarium graminearum hypovirus 1 (FgHV1)", which was closely related to CHV1 and CHV2 in the Hypoviridae family. Elimination of the virus resulted in no dramatic phenotypic alteration of the fungus.
KW - DsRNA
KW - FgHV1
KW - Fusarium graminearum
KW - Fusarium head blight
KW - Hypoviridae
KW - Mycovirus
UR - http://www.scopus.com/inward/record.url?scp=84877607831&partnerID=8YFLogxK
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U2 - 10.1016/j.virusres.2013.03.002
DO - 10.1016/j.virusres.2013.03.002
M3 - Article
C2 - 23499998
AN - SCOPUS:84877607831
SN - 0168-1702
VL - 174
SP - 69
EP - 77
JO - Virus Research
JF - Virus Research
IS - 1-2
ER -
