A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Tetsuro Mazaki; Yasuyuki Shiozaki; Kentarou Yamane; Aki Yoshida; Mariko Nakamura; Yasuhiro Yoshida; Di Zhou; Takashi Kitajima; Masato Tanaka; Yoshihiro Ito; Toshifumi Ozaki; Akihiro Matsukawa

doi:10.1038/srep04457

A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Tetsuro Mazaki, Yasuyuki Shiozaki, Kentarou Yamane, Aki Yoshida, Mariko Nakamura, Yasuhiro Yoshida, Di Zhou, Takashi Kitajima, Masato Tanaka, Yoshihiro Ito, Toshifumi Ozaki, Akihiro Matsukawa

Research output: Contribution to journal › Article › peer-review

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Abstract

Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37°C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.

Original language	English
Article number	4457
Journal	Scientific reports
Volume	4
DOIs	https://doi.org/10.1038/srep04457
Publication status	Published - Mar 25 2014

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@article{eca64f17c92b48ea9e28c1c2e2432918,

title = "A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering",

abstract = "Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37°C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.",

author = "Tetsuro Mazaki and Yasuyuki Shiozaki and Kentarou Yamane and Aki Yoshida and Mariko Nakamura and Yasuhiro Yoshida and Di Zhou and Takashi Kitajima and Masato Tanaka and Yoshihiro Ito and Toshifumi Ozaki and Akihiro Matsukawa",

note = "Funding Information: We thank Dr. Takayuki Furumatsu, Ms. Reina Tanaka, Mr. Yasuharu Arashima, and Mr. Haruyuki Watanabe for their excellent technical assistance. This work was supported in part by 1) grants from Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (S) of KAKENHI 22220009 and (C) of KAKENHI 25462334, and by 2) the Strategic International Research Cooperative Program, Japan Science and Technology Agency (JST), AS232Z00465F.",

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doi = "10.1038/srep04457",

language = "English",

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T1 - A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

AU - Mazaki, Tetsuro

AU - Shiozaki, Yasuyuki

AU - Yamane, Kentarou

AU - Yoshida, Aki

AU - Nakamura, Mariko

AU - Yoshida, Yasuhiro

AU - Zhou, Di

AU - Kitajima, Takashi

AU - Tanaka, Masato

AU - Ito, Yoshihiro

AU - Ozaki, Toshifumi

AU - Matsukawa, Akihiro

N1 - Funding Information: We thank Dr. Takayuki Furumatsu, Ms. Reina Tanaka, Mr. Yasuharu Arashima, and Mr. Haruyuki Watanabe for their excellent technical assistance. This work was supported in part by 1) grants from Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (S) of KAKENHI 22220009 and (C) of KAKENHI 25462334, and by 2) the Strategic International Research Cooperative Program, Japan Science and Technology Agency (JST), AS232Z00465F.

PY - 2014/3/25

Y1 - 2014/3/25

N2 - Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37°C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.

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ER -

A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

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