A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl - secretory pathways to cause diarrhea

Naomi Tanoue; Akira Takahashi; Keinosuke Okamoto; Yoshio Fujii; Yutaka Taketani; Nagakatsu Harada; Masayuki Nakano; Yutaka Nakaya

doi:10.1016/j.femsle.2004.11.009

A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl ^- secretory pathways to cause diarrhea

Naomi Tanoue, Akira Takahashi, Keinosuke Okamoto, Yoshio Fujii, Yutaka Taketani, Nagakatsu Harada, Masayuki Nakano, Yutaka Nakaya

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a human pathogen that causes diarrhea. We investigated the effects of ASH on Cl ^- transport in human colonic epithelial cells. ASH increased short-circuit currents (Isc) and ¹²⁵I efflux from Caco-2 cells, indicating ASH activate Cl ^- secretion. Additions of inhibitors of cyclic AMP dependent Cl ^- channels, glybenclamide and NPPB suppressed the Isc and ¹²⁵I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP-dependent Cl ^- secretory pathway could be related with diarrhea induced by A. sobria.

Original language	English
Pages (from-to)	195-201
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	242
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2004.11.009
Publication status	Published - Jan 15 2005

Keywords

Aeromonas sobria hemolysin
CFTR
Cl secretion
Colonic epithelial cell

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.femsle.2004.11.009

Cite this

@article{de6797dfbe784c46b938aab73dfbe27e,

title = "A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl - secretory pathways to cause diarrhea",

abstract = "Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a human pathogen that causes diarrhea. We investigated the effects of ASH on Cl - transport in human colonic epithelial cells. ASH increased short-circuit currents (Isc) and 125I efflux from Caco-2 cells, indicating ASH activate Cl - secretion. Additions of inhibitors of cyclic AMP dependent Cl - channels, glybenclamide and NPPB suppressed the Isc and 125I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP-dependent Cl - secretory pathway could be related with diarrhea induced by A. sobria.",

keywords = "Aeromonas sobria hemolysin, CFTR, Cl secretion, Colonic epithelial cell",

author = "Naomi Tanoue and Akira Takahashi and Keinosuke Okamoto and Yoshio Fujii and Yutaka Taketani and Nagakatsu Harada and Masayuki Nakano and Yutaka Nakaya",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for scientific research (14770117) from the Ministry of Education, Science, Sports and Culture, Japan to Dr. Akira Takahashi.",

year = "2005",

month = jan,

day = "15",

doi = "10.1016/j.femsle.2004.11.009",

language = "English",

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T1 - A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl - secretory pathways to cause diarrhea

AU - Tanoue, Naomi

AU - Takahashi, Akira

AU - Okamoto, Keinosuke

AU - Fujii, Yoshio

AU - Taketani, Yutaka

AU - Harada, Nagakatsu

AU - Nakano, Masayuki

AU - Nakaya, Yutaka

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for scientific research (14770117) from the Ministry of Education, Science, Sports and Culture, Japan to Dr. Akira Takahashi.

PY - 2005/1/15

Y1 - 2005/1/15

N2 - Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a human pathogen that causes diarrhea. We investigated the effects of ASH on Cl - transport in human colonic epithelial cells. ASH increased short-circuit currents (Isc) and 125I efflux from Caco-2 cells, indicating ASH activate Cl - secretion. Additions of inhibitors of cyclic AMP dependent Cl - channels, glybenclamide and NPPB suppressed the Isc and 125I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP-dependent Cl - secretory pathway could be related with diarrhea induced by A. sobria.

AB - Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a human pathogen that causes diarrhea. We investigated the effects of ASH on Cl - transport in human colonic epithelial cells. ASH increased short-circuit currents (Isc) and 125I efflux from Caco-2 cells, indicating ASH activate Cl - secretion. Additions of inhibitors of cyclic AMP dependent Cl - channels, glybenclamide and NPPB suppressed the Isc and 125I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP-dependent Cl - secretory pathway could be related with diarrhea induced by A. sobria.

KW - Aeromonas sobria hemolysin

KW - CFTR

KW - Cl secretion

KW - Colonic epithelial cell

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