A Potent Insect Chitinase Inhibitor of Fungal Origin

Teruhiko Nitoda; Hirokazu Usuki; Hiroshi Kanzaki

doi:10.1515/znc-2003-11-1226

A Potent Insect Chitinase Inhibitor of Fungal Origin

Teruhiko Nitoda, Hirokazu Usuki, Hiroshi Kanzaki

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC₅₀ value of 28 nM.

Original language	English
Pages (from-to)	891-894
Number of pages	4
Journal	Zeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume	58
Issue number	11-12
DOIs	https://doi.org/10.1515/znc-2003-11-1226
Publication status	Published - 2003

Keywords

Chitinase Inhibitor
Extracellular Polysaccharide
Sphaeropsis sp.

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1515/znc-2003-11-1226

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abstract = "A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC50 value of 28 nM.",

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AU - Nitoda, Teruhiko

AU - Usuki, Hirokazu

AU - Kanzaki, Hiroshi

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N2 - A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC50 value of 28 nM.

AB - A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC50 value of 28 nM.

KW - Chitinase Inhibitor

KW - Extracellular Polysaccharide

KW - Sphaeropsis sp.

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A Potent Insect Chitinase Inhibitor of Fungal Origin

Abstract

Keywords

ASJC Scopus subject areas

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