TY - JOUR
T1 - A proteome analysis of pig pancreatic islets and exocrine tissue by liquid chromatography with tandem mass spectrometry
AU - Nakashima, Yoshiki
AU - Miyagi-Shiohira, Chika
AU - Kobayashi, Naoya
AU - Saitoh, Issei
AU - Watanabe, Masami
AU - Noguchi, Hirofumi
N1 - Funding Information:
This work was supported in part by the Japan Society for the Promotion of Science, Japan Agency for Medical Research and Development, The Naito Foundation, and Okinawa Science and Technology Promotion Center (OSTC).
Funding Information:
We thank Ms. Naomi Kakazu (University of the Ryukyus) for the office processing and Ms. Saki Uema, Chie Horiguchi, Yuka Watanabe and Maki Higa (University of the Ryukyus) for technical support. This work was supported in part by the Japan Society for the Promotion of Science, Japan Agency for Medical Research and Development, The Naito Foundation, and Okinawa Science and Technology Promotion Center (OSTC).
Publisher Copyright:
© 2017 Taylor & Francis Group, LLC.
PY - 2017/11/2
Y1 - 2017/11/2
N2 - Liquid chromatography with tandem mass spectrometry (LC-MS/MS) is a proteome analysis method, and the shotgun analysis by LC-MS/MS comprehensively identifies proteins from tissues and cells with high resolving power. In this study, we analyzed the protein expression in pancreatic tissue by LC-MS/MS. Islets isolated from porcine pancreata (purity ≥95%) and exocrine tissue (purity ≥99%) were used in this study. LC-MS/MS showed that 13 proteins were expressed in pancreatic islets only (Group I), 43 proteins were expressed in both islets and exocrine tissue (Group I&E), and 102 proteins were expressed in exocrine tissue only (Group E). Proteins involved in islet differentiation and cell proliferation were identified in Group I (e.g. CLUS, CMGA, MIF). In addition, various functional proteins (e.g. SCG2, TBA1A) were identified in islet by using the new method of ‘principal component analysis (PCA)’. However, the function of such proteins on islets remains unclear. EPCAM was identified in Group E. Group E was found to include proteins involved in clinical inflammatory diseases such as pancreatitis (e.g. CBPA1, CGL, CYTB, ISK1 and PA21B). Many of these identified proteins were reported less frequently in previous studies, and HS71B, NEC2, PRAF3 and SCG1 were newly detected in Group I while CPNS1, DPEP1, GANAB, GDIB, GGT1, HSPB1, ICTL, VILI, MUTA, NDKB, PTGR1, UCHL3, VAPB and VINC were newly detected in Group E. These results show that comprehensive expression analysis of proteins by LC-MS/MS is useful as a method to investigate new factors constructing cellular component, biological process, and molecular function.
AB - Liquid chromatography with tandem mass spectrometry (LC-MS/MS) is a proteome analysis method, and the shotgun analysis by LC-MS/MS comprehensively identifies proteins from tissues and cells with high resolving power. In this study, we analyzed the protein expression in pancreatic tissue by LC-MS/MS. Islets isolated from porcine pancreata (purity ≥95%) and exocrine tissue (purity ≥99%) were used in this study. LC-MS/MS showed that 13 proteins were expressed in pancreatic islets only (Group I), 43 proteins were expressed in both islets and exocrine tissue (Group I&E), and 102 proteins were expressed in exocrine tissue only (Group E). Proteins involved in islet differentiation and cell proliferation were identified in Group I (e.g. CLUS, CMGA, MIF). In addition, various functional proteins (e.g. SCG2, TBA1A) were identified in islet by using the new method of ‘principal component analysis (PCA)’. However, the function of such proteins on islets remains unclear. EPCAM was identified in Group E. Group E was found to include proteins involved in clinical inflammatory diseases such as pancreatitis (e.g. CBPA1, CGL, CYTB, ISK1 and PA21B). Many of these identified proteins were reported less frequently in previous studies, and HS71B, NEC2, PRAF3 and SCG1 were newly detected in Group I while CPNS1, DPEP1, GANAB, GDIB, GGT1, HSPB1, ICTL, VILI, MUTA, NDKB, PTGR1, UCHL3, VAPB and VINC were newly detected in Group E. These results show that comprehensive expression analysis of proteins by LC-MS/MS is useful as a method to investigate new factors constructing cellular component, biological process, and molecular function.
KW - LC-MS/MS analysis
KW - cell isolation
KW - exocrine tissue
KW - islet
KW - pancreatic
KW - pig
UR - http://www.scopus.com/inward/record.url?scp=85032801757&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032801757&partnerID=8YFLogxK
U2 - 10.1080/19382014.2017.1389826
DO - 10.1080/19382014.2017.1389826
M3 - Article
C2 - 29099648
AN - SCOPUS:85032801757
SN - 1938-2014
VL - 9
SP - 159
EP - 176
JO - Islets
JF - Islets
IS - 6
ER -
