TY - JOUR
T1 - A sensitive assay for mutagenic activity of N-nitrosamines and its use for detection of modulators of the mutagenicity.
AU - Negishi, T.
AU - Ohara, Y.
AU - Hayatsu, H.
PY - 1982/12/1
Y1 - 1982/12/1
N2 - By carrying out the pre-incubation of bacteria with N-nitrosamines under acidic conditions, the mutagenicity of N-nitrosodimethylamine on Salmonella TA100 and E. coli WP2 try, hcr was detected with increased sensitivity, compared to usual assays in neutral buffers. Using this modified assay system, compounds have been screened for their modulating effects on N-nitrosamine-mediated mutagenesis and the following were found to be inhibitors: cysteine, cysteamine, bisulfite, n-propylamine, n-butylamine, n-hexylamine, n-octylamine, n-nonylamine, serotonine, tryptamine, epinephrine, norepinephrine and oleic acid. The mechanisms of inhibition are discussed.
AB - By carrying out the pre-incubation of bacteria with N-nitrosamines under acidic conditions, the mutagenicity of N-nitrosodimethylamine on Salmonella TA100 and E. coli WP2 try, hcr was detected with increased sensitivity, compared to usual assays in neutral buffers. Using this modified assay system, compounds have been screened for their modulating effects on N-nitrosamine-mediated mutagenesis and the following were found to be inhibitors: cysteine, cysteamine, bisulfite, n-propylamine, n-butylamine, n-hexylamine, n-octylamine, n-nonylamine, serotonine, tryptamine, epinephrine, norepinephrine and oleic acid. The mechanisms of inhibition are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0020388976&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020388976&partnerID=8YFLogxK
M3 - Article
C2 - 6754597
AN - SCOPUS:0020388976
SN - 0300-5038
SP - 685
EP - 694
JO - IARC scientific publications
JF - IARC scientific publications
IS - 41
ER -