A Sensitive Spectrofluorimetric Method for the Determination of Human Serum Albumin with Chrome-azurol S

Yutaka Saito; Yukiko Okazaki; Shoko Yano; Masaki Mifune; Junichi Odo; Yoshimasa Tanaka; Atsuko Kanetsuna; Kei Ichi Miyazaki

doi:10.1248/cpb.34.2880

A Sensitive Spectrofluorimetric Method for the Determination of Human Serum Albumin with Chrome-azurol S

Yutaka Saito, Yukiko Okazaki, Shoko Yano, Masaki Mifune, Junichi Odo, Yoshimasa Tanaka, Atsuko Kanetsuna, Kei Ichi Miyazaki

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

An extremely sensitive spectrofluorimetric method for assay of human serum albumin (HSA) was developed, based on binding of chrome-azurol S(CAS) by HSA. The CAS-HSA complex shows a fluorescence at 616nm, whereas CAS alone exhibits only a weak fluorescence at this wavelength. A nonionic detergent, polyethylene glycol-p-nonylphenyl ether n=10, acts as a stabilizer of the CAS-HSA complex. The calibration curve was linear in the range from 5 to 80 μg of HSA in the final solution. Relative standard deviation was 1.3% (n=10). The method requires less than one-thirtieth of the amount of HSA required by the control bromcresol green method. HSA values in control sera and spinal fluid assayed by the new method were in agreement with those obtained by the control bromcresol green method.

Original language	English
Pages (from-to)	2880-2884
Number of pages	5
Journal	Chemical and Pharmaceutical Bulletin
Volume	34
Issue number	7
DOIs	https://doi.org/10.1248/cpb.34.2880
Publication status	Published - 1986

Keywords

Albumin chrome-azurol S complex
Albumin sensitive determination
Albumin spectrofluorimetry
Chrome-azurol S
Dye binding method
Spinal fluid albumin

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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title = "A Sensitive Spectrofluorimetric Method for the Determination of Human Serum Albumin with Chrome-azurol S",

abstract = "An extremely sensitive spectrofluorimetric method for assay of human serum albumin (HSA) was developed, based on binding of chrome-azurol S(CAS) by HSA. The CAS-HSA complex shows a fluorescence at 616nm, whereas CAS alone exhibits only a weak fluorescence at this wavelength. A nonionic detergent, polyethylene glycol-p-nonylphenyl ether n=10, acts as a stabilizer of the CAS-HSA complex. The calibration curve was linear in the range from 5 to 80 μg of HSA in the final solution. Relative standard deviation was 1.3% (n=10). The method requires less than one-thirtieth of the amount of HSA required by the control bromcresol green method. HSA values in control sera and spinal fluid assayed by the new method were in agreement with those obtained by the control bromcresol green method.",

keywords = "Albumin chrome-azurol S complex, Albumin sensitive determination, Albumin spectrofluorimetry, Chrome-azurol S, Dye binding method, Spinal fluid albumin",

author = "Yutaka Saito and Yukiko Okazaki and Shoko Yano and Masaki Mifune and Junichi Odo and Yoshimasa Tanaka and Atsuko Kanetsuna and Miyazaki, {Kei Ichi}",

year = "1986",

doi = "10.1248/cpb.34.2880",

language = "English",

volume = "34",

pages = "2880--2884",

journal = "Chemical and Pharmaceutical Bulletin",

issn = "0009-2363",

publisher = "Pharmaceutical Society of Japan",

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T1 - A Sensitive Spectrofluorimetric Method for the Determination of Human Serum Albumin with Chrome-azurol S

AU - Saito, Yutaka

AU - Okazaki, Yukiko

AU - Yano, Shoko

AU - Mifune, Masaki

AU - Odo, Junichi

AU - Tanaka, Yoshimasa

AU - Kanetsuna, Atsuko

AU - Miyazaki, Kei Ichi

PY - 1986

Y1 - 1986

N2 - An extremely sensitive spectrofluorimetric method for assay of human serum albumin (HSA) was developed, based on binding of chrome-azurol S(CAS) by HSA. The CAS-HSA complex shows a fluorescence at 616nm, whereas CAS alone exhibits only a weak fluorescence at this wavelength. A nonionic detergent, polyethylene glycol-p-nonylphenyl ether n=10, acts as a stabilizer of the CAS-HSA complex. The calibration curve was linear in the range from 5 to 80 μg of HSA in the final solution. Relative standard deviation was 1.3% (n=10). The method requires less than one-thirtieth of the amount of HSA required by the control bromcresol green method. HSA values in control sera and spinal fluid assayed by the new method were in agreement with those obtained by the control bromcresol green method.

AB - An extremely sensitive spectrofluorimetric method for assay of human serum albumin (HSA) was developed, based on binding of chrome-azurol S(CAS) by HSA. The CAS-HSA complex shows a fluorescence at 616nm, whereas CAS alone exhibits only a weak fluorescence at this wavelength. A nonionic detergent, polyethylene glycol-p-nonylphenyl ether n=10, acts as a stabilizer of the CAS-HSA complex. The calibration curve was linear in the range from 5 to 80 μg of HSA in the final solution. Relative standard deviation was 1.3% (n=10). The method requires less than one-thirtieth of the amount of HSA required by the control bromcresol green method. HSA values in control sera and spinal fluid assayed by the new method were in agreement with those obtained by the control bromcresol green method.

KW - Albumin chrome-azurol S complex

KW - Albumin sensitive determination

KW - Albumin spectrofluorimetry

KW - Chrome-azurol S

KW - Dye binding method

KW - Spinal fluid albumin

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SN - 0009-2363

VL - 34

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EP - 2884

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

IS - 7

ER -

A Sensitive Spectrofluorimetric Method for the Determination of Human Serum Albumin with Chrome-azurol S

Abstract

Keywords

ASJC Scopus subject areas

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