A Sensitive Spectrophotometric Method for the Determination of Human Serum Albumin with Chrome-azurol S Aluminium Chelate

A simple and highly sensitive method for the determination of serum albumin based on the formation of a ternary complex from chrome-azurol S aluminium chelate was developed. A nonionic detergent, polyethylene glycol-p-nonylphenyl ether n=10, acted as a stabilizer of the ternary complex in the present system. The formation of the ternary complex caused a large increase in absorbance at 630 nm, and the calibration curve was linear between 10 and 200 μg of albumin in the final colored solution (8.0 ml). The sensitivity of the present method was about eight times that of a bromcresol green control method. Heparin and bilirubin did not interfere with the determination.