TY - JOUR
T1 - A simple, rapid method for gizzerosine analysis in fish meal by paper electrophoresis
AU - Tao, Zhihua
AU - Sato, Minoru
AU - Wu, Kegang
AU - Kiyota, Hiromasa
AU - Yamaguchi, Toshiyasu
AU - Nakano, Toshiki
N1 - Funding Information:
Acknowledgments This work was partly supported by Project 211 of Guangdong Province of China (no. 20091015) and the National Natural Science Foundation of China (no. 31101743).
PY - 2012/7
Y1 - 2012/7
N2 - We established a simple, rapid method for gizzerosine analysis in fish meal. Gizzerosine was extracted from fish meal with 0.1 N HCl solution. Samples and standard gizzerosine solutions were absorbed onto a paper disc, which was then set on electrophoresis paper for 18 min, and the paper was dried. Gizzerosine was visualized with Pauly's reagent, and the intensity of the colored spots was digitized and calculated by image processing method software. We achieved successful separation of gizzerosine from other Pauly's reagent-positive components in fish meal extracts. The linearity of gizzerosine estimation using this method was within the range 30-1000 ng (R 2 = 0.99). Gizzerosine was satisfactorily detected and completely separated from histamine and other Pauly's reagent-positive compounds. This method does not require expensive instruments or tedious pretreatment to eliminate interfering compounds, such as histamine or histidine. It also uses less reagent compared with high-performance liquid chromatography. Moreover, it is a simple, rapid, sensitive, and reproducible method. It is suitable for monitoring gizzerosine in fish meal products that contain as little as 10 ppm gizzerosine.
AB - We established a simple, rapid method for gizzerosine analysis in fish meal. Gizzerosine was extracted from fish meal with 0.1 N HCl solution. Samples and standard gizzerosine solutions were absorbed onto a paper disc, which was then set on electrophoresis paper for 18 min, and the paper was dried. Gizzerosine was visualized with Pauly's reagent, and the intensity of the colored spots was digitized and calculated by image processing method software. We achieved successful separation of gizzerosine from other Pauly's reagent-positive components in fish meal extracts. The linearity of gizzerosine estimation using this method was within the range 30-1000 ng (R 2 = 0.99). Gizzerosine was satisfactorily detected and completely separated from histamine and other Pauly's reagent-positive compounds. This method does not require expensive instruments or tedious pretreatment to eliminate interfering compounds, such as histamine or histidine. It also uses less reagent compared with high-performance liquid chromatography. Moreover, it is a simple, rapid, sensitive, and reproducible method. It is suitable for monitoring gizzerosine in fish meal products that contain as little as 10 ppm gizzerosine.
KW - Determination
KW - Fish
KW - Gizzerosine
KW - Paper electrophoresis
KW - Pauly's reagent
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U2 - 10.1007/s12562-012-0507-3
DO - 10.1007/s12562-012-0507-3
M3 - Article
AN - SCOPUS:84864075902
SN - 0919-9268
VL - 78
SP - 923
EP - 926
JO - Fisheries Science
JF - Fisheries Science
IS - 4
ER -