A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

Kanae Muraiso; Makoto Hijikata; Showgo Ohkoshi; Myung Je Cho; Masayoshi Kikuchi; Nobuyuki Kato; Kunitada Shimotohno

doi:10.1016/0006-291X(90)90702-O

A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

Kanae Muraiso, Makoto Hijikata, Showgo Ohkoshi, Myung Je Cho, Masayoshi Kikuchi, Nobuyuki Kato, Kunitada Shimotohno

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.

Original language	English
Pages (from-to)	511-516
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	172
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)90702-O
Publication status	Published - Oct 30 1990
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0006-291X(90)90702-O

Cite this

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title = "A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus",

abstract = "A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.",

author = "Kanae Muraiso and Makoto Hijikata and Showgo Ohkoshi and Cho, {Myung Je} and Masayoshi Kikuchi and Nobuyuki Kato and Kunitada Shimotohno",

note = "Funding Information: ACKNOWLEDGMENTS: This study was supported by Grants-in-Aid Cancer Research and for a Comprehensive lo-Year Strategy Cancer Control from the Ministry of Health and Welfare, K.M. and M.H. are the recipients of Research Resident Fellowships from the Foundation for Promotion of Cancer Research. We thank M. Ohara for helpful comments.",

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doi = "10.1016/0006-291X(90)90702-O",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

AU - Muraiso, Kanae

AU - Hijikata, Makoto

AU - Ohkoshi, Showgo

AU - Cho, Myung Je

AU - Kikuchi, Masayoshi

AU - Kato, Nobuyuki

AU - Shimotohno, Kunitada

N1 - Funding Information: ACKNOWLEDGMENTS: This study was supported by Grants-in-Aid Cancer Research and for a Comprehensive lo-Year Strategy Cancer Control from the Ministry of Health and Welfare, K.M. and M.H. are the recipients of Research Resident Fellowships from the Foundation for Promotion of Cancer Research. We thank M. Ohara for helpful comments.

PY - 1990/10/30

Y1 - 1990/10/30

N2 - A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.

AB - A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.

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A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

Abstract

ASJC Scopus subject areas

UN SDGs

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