@article{4c941e3e7b584985843a6247b09d6cbd,
title = "A transcription factor OsbHLH156 regulates Strategy II iron acquisition through localising IRO2 to the nucleus in rice",
abstract = "Plants have evolved two strategies to acquire ferrous (Strategy I) or ferric (Strategy II) iron from soil. The iron-related bHLH transcription factor 2 (IRO2) has been identified as a key regulator of iron acquisition (Strategy II) in rice. However, its mode of action, subcellular localisation and binding partners are not clearly defined. Using RNA-seq analyses, we identified a novel bHLH-type transcription factor, OsbHLH156. The function of OsbHLH156 in Fe homeostasis was analysed by characterisation of the phenotypes, elemental content, transcriptome, interaction and subcellular localisation of OsbHLH156 and IRO2. OsbHLH156 is primarily expressed in the roots and transcript abundance is greatly increased by Fe deficiency. Loss of function of OsbHLH156 resulted in Fe-deficiency-induced chlorosis and reduced Fe concentration in the shoots under upland or Fe(III) supplied conditions. Transcriptome analyses revealed that the expression of most Fe-deficiency-responsive genes involved in Strategy II were not induced in the osbhlh156-1 mutant. Furthermore, OsbHLH156 was required for nuclear localisation of IRO2. We conclude that OsbHLH156 is required for a Strategy II uptake mechanism in rice, partnering with a previously identified {\textquoteleft}master{\textquoteright} regulator IRO2. Mechanistically it is required for the nuclear localisation of IRO2.",
keywords = "Fe deficiency, nuclear localisation, protein interaction, rice, transcription factor",
author = "Shoudong Wang and Lin Li and Yinghui Ying and Jin Wang and Shao Jifeng and Naoki Yamaji and James Whelan and Ma, {Jian Feng} and Huixia Shou",
note = "Funding Information: The authors would like to thank Luis Herrera-Estrella of the Laboratorio Nacional de Gen?mica para la Biodiversidad (LANGEBIO), Mexico for providing productive discussion. We also thank Heven Sze (University of Maryland, College Park, MD, USA) and Mary Williams (Editor of The Plant Cell), for critical comments and manuscript editing. Shelong Zhang provided technical support in the use of fluorescence microscope in the study. This work was supported by the Ministry of Science and Technology of China (2016YFD0100703), the National Natural Science Foundation of China (31401934, 31572189 and 31771689), the Natural Science Foundation of Zhejiang Province (LZ16C 150001), the Ministry of Education (B14027), the Australian Research Council Centre of Excellence Program (Grant no. CE140100008), and by Grant-in-Aid for Specially Promoted Research (JSPS KAKENHI Grant no. 16H06296 to JFM). Funding Information: The authors would like to thank Luis Herrera‐Estrella of the Laboratorio Nacional de Gen{\'o}mica para la Biodiversidad (LANGEBIO), Mexico for providing productive discussion. We also thank Heven Sze (University of Maryland, College Park, MD, USA) and Mary Williams (Editor of The Plant Cell), for critical comments and manuscript editing. Shelong Zhang provided technical support in the use of fluorescence microscope in the study. This work was supported by the Ministry of Science and Technology of China (2016YFD0100703), the National Natural Science Foundation of China (31401934, 31572189 and 31771689), the Natural Science Foundation of Zhejiang Province (LZ16C 150001), the Ministry of Education (B14027), the Australian Research Council Centre of Excellence Program (Grant no. CE140100008), and by Grant‐in‐Aid for Specially Promoted Research (JSPS KAKENHI Grant no. 16H06296 to JFM). Publisher Copyright: {\textcopyright} 2019 The Authors. New Phytologist {\textcopyright} 2019 New Phytologist Trust",
year = "2020",
month = feb,
day = "1",
doi = "10.1111/nph.16232",
language = "English",
volume = "225",
pages = "1247--1260",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "3",
}