TY - JOUR
T1 - A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
AU - Kato, Koji
AU - Tanaka, Hideaki
AU - Sumizawa, Tomoyuki
AU - Yoshimura, Masato
AU - Yamashita, Eiki
AU - Iwasaki, Kenji
AU - Tsukihara, Tomitake
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/4/19
Y1 - 2008/4/19
N2 - Vault is a 12.9 MDa ribonucleoprotein particle with a barrel-like shape, two protruding caps and an invaginated waist structure that is highly conserved in a wide variety of eukaryotes. Multimerization of the major vault protein (MVP) is sufficient to assemble the entire exterior shell of the barrel-shaped vault particle. Multiple copies of two additional proteins, vault poly(ADP-ribose) polymerase (VPARP) and telomerase-associated protein 1 (TEP1), as well as a small vault RNA (vRNA), are also associated with vault. Here, the crystallization of vault particles is reported. The crystals belong to space group C2, with unit-cell parameters a = 708.0, b = 385.0, c = 602.9 Å, β = 124.8°. Rotational symmetry searches based on the R factor and correlation coefficient from noncrystallographic symmetry (NCS) averaging indicated that the particle has 39-fold dihedral symmetry.
AB - Vault is a 12.9 MDa ribonucleoprotein particle with a barrel-like shape, two protruding caps and an invaginated waist structure that is highly conserved in a wide variety of eukaryotes. Multimerization of the major vault protein (MVP) is sufficient to assemble the entire exterior shell of the barrel-shaped vault particle. Multiple copies of two additional proteins, vault poly(ADP-ribose) polymerase (VPARP) and telomerase-associated protein 1 (TEP1), as well as a small vault RNA (vRNA), are also associated with vault. Here, the crystallization of vault particles is reported. The crystals belong to space group C2, with unit-cell parameters a = 708.0, b = 385.0, c = 602.9 Å, β = 124.8°. Rotational symmetry searches based on the R factor and correlation coefficient from noncrystallographic symmetry (NCS) averaging indicated that the particle has 39-fold dihedral symmetry.
KW - Ribonucleoproteins
KW - Vault
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U2 - 10.1107/S0907444908004277
DO - 10.1107/S0907444908004277
M3 - Article
C2 - 18453688
AN - SCOPUS:43249130948
SN - 0907-4449
VL - 64
SP - 525
EP - 531
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
IS - 5
ER -