Absorption and metabolism of 4-hydroxyderricin and xanthoangelol after oral administration of Angelica keiskei (Ashitaba) extract in mice

Toshiyuki Nakamura, Takahiro Tokushima, Kyuichi Kawabata, Norio Yamamoto, Masaaki Miyamoto, Hitoshi Ashida

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


To investigate the absorption and metabolism of 4-hydroxyderricin and xanthoangelol, we established an analytical method based on liquid chromatography-tandem mass spectrometry and measured these compounds in the plasma, urine, feces, liver, kidney, spleen, muscle and white adipose tissues of mice orally administered with Ashitaba extract (50-500 mg/kg body weight). 4-Hydroxyderricin and xanthoangelol were quickly absorbed into the plasma, with time-to-maximum plasma concentrations of 2 and 0.5 h for 4-hydroxyderricin and xanthoangelol, respectively. Although these compounds have similar structures, the total plasma concentration of 4-hydroxyderricin and its metabolites was approximately 4-fold greater than that of xanthoangelol and its metabolites at 24 h. 4-Hydroxyderricin and xanthoangelol were mostly excreted in their aglycone forms and related metabolites (glucuronate and/or sulfate forms) in urine between 2 and 4 h after oral administration of Ashitaba extract. On the other hand, these compounds were only excreted in their aglycone forms in feces. When tissue distribution of 4-hydroxyderricin and xanthoangelol was estimated 2 h after administration of Ashitaba extract, both compounds were detected in all of the tissues assessed, mainly in their aglycone forms, except in the mesenteric adipose tissue. These results suggest that 4-hydroxyderricin and xanthoangelol are rapidly absorbed and distributed to various tissues.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalArchives of Biochemistry and Biophysics
Issue number1-2
Publication statusPublished - May 2012
Externally publishedYes


  • 4-Hydroxyderricin
  • Absorption
  • Angelica keiskei Koidzumi (Ashitaba)
  • Chalcone
  • Metabolism
  • Xanthoangelol

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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