Accumulation and recovery of copper from minning waste water by Chattonella antiqua

The toxity of cations on Chattonella antiqua was arranged in decreasing order : Cu>Pb>Cr>Pb> Sb, Bi>Ni>Co>V>Mo>Se, As>Ga>Sr>Li, B. Copper accumulated in Chattonella antiqua increased in proportion to the amount of copper ion in medium. The boling water-treated cells accumulated copper ion rapidly than living cells. A method was described to collect the copper ion in minning wastewater with the boiling water-treated powder algae (BWP) and 5 %KOH treated powder algae (ABWP) of Chattonella antiqua, Dunaliellasalina and Synechococcus sp. Miami BG45311. A 50 ml solution containning Cu²⁺ was mixed with 100 mg of the BWP and ABWP under stirring for 30 min. The solution was then centrifuged and filtered through a sintered-glassfilter (0.2 μm) and the concentrations of copper ion in thefiltrate were determined with an atomic absorption spectrometer. The amount of copper accumlated in the BWP and ABWP became a maximum at 30 min after exposure. The ability to accumulate copper was species specific and found tobe (increasing order); ABWP-C. antiqua>BWP-C. antiqua>BWP-S. sp. Miami>BWP-D. salina. The amount of copper accumulated in the BWP of C. antiqua increased with an increase of the amount of algae (0-20 mg dry algae/10 ml) in minning waste water.