Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants

Bunichi Ezaki; Mayandi Sivaguru; Yuka Ezaki; Hideaki Matsumoto; Richard C. Gardner

doi:10.1016/S0378-1097(98)00575-8

Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants

Bunichi Ezaki, Mayandi Sivaguru, Yuka Ezaki, Hideaki Matsumoto, Richard C. Gardner

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Eleven aluminum stress-induced genes derived from plants (wheat, Arabidopsis and tobacco) were introduced into Saccharomyces cerevisiae to test if expression of these genes confers A1 tolerance. A1 sensitivity tests showed that expression of two genes, either an Arabidopsis gene for blue copper binding protein (BCB), or a tobacco gene for the GDP dissociation inhibitor (NtGDI1), conferred A1 tolerance. Determinations of total content and localization of A1 ions in these transformants suggested that the BCB gene product functions in restricting A1 uptake, while expression of the NtGDI1 gene promotes release of A1 ions after uptake.

Original language	English
Pages (from-to)	81-87
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	171
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(98)00575-8
Publication status	Published - Feb 15 1999

Keywords

Aluminum metal stress
BCB gene
Morin
NtGDI1 gene
Tolerant mechanism
Vesicle transport

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1016/S0378-1097(98)00575-8

Cite this

@article{47344f603fcc40d1ad607a3c4c9429eb,

title = "Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants",

abstract = "Eleven aluminum stress-induced genes derived from plants (wheat, Arabidopsis and tobacco) were introduced into Saccharomyces cerevisiae to test if expression of these genes confers A1 tolerance. A1 sensitivity tests showed that expression of two genes, either an Arabidopsis gene for blue copper binding protein (BCB), or a tobacco gene for the GDP dissociation inhibitor (NtGDI1), conferred A1 tolerance. Determinations of total content and localization of A1 ions in these transformants suggested that the BCB gene product functions in restricting A1 uptake, while expression of the NtGDI1 gene promotes release of A1 ions after uptake.",

keywords = "Aluminum metal stress, BCB gene, Morin, NtGDI1 gene, Tolerant mechanism, Vesicle transport",

author = "Bunichi Ezaki and Mayandi Sivaguru and Yuka Ezaki and Hideaki Matsumoto and Gardner, {Richard C.}",

note = "Funding Information: This work received financial support from the Program for Promotion of Basic Research Activities for Innovative Biosciences, the New Zealand Foundation for Research Science and Technology (96-AGR-03-5253), Core Research for Evolutional Science and Technology of Japan Science and Technology Corporation, and the Japan Society for Promotion of Science.",

year = "1999",

month = feb,

day = "15",

doi = "10.1016/S0378-1097(98)00575-8",

language = "English",

volume = "171",

pages = "81--87",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants

AU - Ezaki, Bunichi

AU - Sivaguru, Mayandi

AU - Ezaki, Yuka

AU - Matsumoto, Hideaki

AU - Gardner, Richard C.

N1 - Funding Information: This work received financial support from the Program for Promotion of Basic Research Activities for Innovative Biosciences, the New Zealand Foundation for Research Science and Technology (96-AGR-03-5253), Core Research for Evolutional Science and Technology of Japan Science and Technology Corporation, and the Japan Society for Promotion of Science.

PY - 1999/2/15

Y1 - 1999/2/15

N2 - Eleven aluminum stress-induced genes derived from plants (wheat, Arabidopsis and tobacco) were introduced into Saccharomyces cerevisiae to test if expression of these genes confers A1 tolerance. A1 sensitivity tests showed that expression of two genes, either an Arabidopsis gene for blue copper binding protein (BCB), or a tobacco gene for the GDP dissociation inhibitor (NtGDI1), conferred A1 tolerance. Determinations of total content and localization of A1 ions in these transformants suggested that the BCB gene product functions in restricting A1 uptake, while expression of the NtGDI1 gene promotes release of A1 ions after uptake.

AB - Eleven aluminum stress-induced genes derived from plants (wheat, Arabidopsis and tobacco) were introduced into Saccharomyces cerevisiae to test if expression of these genes confers A1 tolerance. A1 sensitivity tests showed that expression of two genes, either an Arabidopsis gene for blue copper binding protein (BCB), or a tobacco gene for the GDP dissociation inhibitor (NtGDI1), conferred A1 tolerance. Determinations of total content and localization of A1 ions in these transformants suggested that the BCB gene product functions in restricting A1 uptake, while expression of the NtGDI1 gene promotes release of A1 ions after uptake.

KW - Aluminum metal stress

KW - BCB gene

KW - Morin

KW - NtGDI1 gene

KW - Tolerant mechanism

KW - Vesicle transport

UR - http://www.scopus.com/inward/record.url?scp=0033005739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033005739&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(98)00575-8

DO - 10.1016/S0378-1097(98)00575-8

M3 - Article

C2 - 10077831

AN - SCOPUS:0033005739

SN - 0378-1097

VL - 171

SP - 81

EP - 87

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this