TY - JOUR
T1 - Acridine orange as a fluorescent probe for lysosomal proton pump
AU - Moriyama, Yoshinori
AU - Takano, Tatsuya
AU - Ohkuma, Shoji
PY - 1982/10
Y1 - 1982/10
N2 - Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the ΔpH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dicyclohexylcar-bodiimide, N-ethylmaleimide, and azide, but not to oligomycin, ouabain or vanadate. These results supported the idea of the existence of a lysosomal H+-pump, suggested in a previous paper (Ohkuma et al. (1982) Proc. Natl. Acad. Sci. U.S. 79, 2758-2762).
AB - Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the ΔpH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dicyclohexylcar-bodiimide, N-ethylmaleimide, and azide, but not to oligomycin, ouabain or vanadate. These results supported the idea of the existence of a lysosomal H+-pump, suggested in a previous paper (Ohkuma et al. (1982) Proc. Natl. Acad. Sci. U.S. 79, 2758-2762).
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U2 - 10.1093/oxfordjournals.jbchem.a134053
DO - 10.1093/oxfordjournals.jbchem.a134053
M3 - Article
C2 - 6294070
AN - SCOPUS:0020195691
SN - 0021-924X
VL - 92
SP - 1333
EP - 1336
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 4
ER -