Actions of gluco-oligosaccharide against Staphylococcus aureus

Hisanori Akiyama, Takashi Oono, Wook Kang Huh, Osamu Yamasaki, Yukie Akagi, Hideto Uemura, Tetsumasa Yamada, Keiji Iwatsuki

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17 Citations (Scopus)


Heavy colonization of atopic dermatitis (AD) with Staphylococcus aureus is well documented. The purpose of the present study is to examine the actions of gluco-oligosaccharide (G-OS) against S. aureus for the control of AD skin lesions infected with S. aureus. The colony counts of S. aureus cells in 0.5% sodium chloride solution supplemented with 5% G-OS (pH 4.8) were about 10-fold lower than those in 0.5% sodium chloride solution (pH 6.6; control) after incubation for 24 hours. The colony counts of S. aureus cells attached on the coverslips (pre-treatment with 1% and 5% G-OS/PBS and following treatment with plasma) were about 10-fold lower than those on the coverslips (pre-treatment with PBS and following treatment with plasma; control) in PBS after incubation for 24 hours. The materials (sugars, probably glycocalyx) that stained positively for fluorescein-isothiocyanate (FITC)-concanavalin A and were consistent with the presence of S. aureus cells were reduced when S. aureus cells attached to the coverslips treated with 5% G-OS. In conclusion, G-OS is a promising agent that can be applied topically in a cream to clear adherent S. aureus cells from skin lesions of AD in order to prevent its exacerbation. Further, 5% G-OS can inhibit glycocalyx production by S. aureus cells and consequently have some suppressive effect on the colonization of S. aureus on the horny cells of AD lesions.

Original languageEnglish
Pages (from-to)580-586
Number of pages7
JournalJournal of Dermatology
Issue number9
Publication statusPublished - Sept 1 2002


  • Attachment
  • Gluco-oligosaccharide
  • Glycocalyx production
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Dermatology


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