Activation of protease by sol-gel entrapment into organically modified hybrid silicates

Shin Ya Furukawa; Tsutomu Ono; Hiroyuki Ijima; Koei Kawakami

doi:10.1023/A:1013876313806

Activation of protease by sol-gel entrapment into organically modified hybrid silicates

Shin Ya Furukawa, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.

Original language	English
Pages (from-to)	13-16
Number of pages	4
Journal	Biotechnology Letters
Volume	24
Issue number	1
DOIs	https://doi.org/10.1023/A:1013876313806
Publication status	Published - 2002
Externally published	Yes

Keywords

Organic solvent
Organic-inorganic hybrid silicate
Protease
Sol-gel method
Transesterification

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1023/A:1013876313806

Cite this

@article{09201eb30ff64b329bf17a94fe5fee37,

title = "Activation of protease by sol-gel entrapment into organically modified hybrid silicates",

abstract = "To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.",

keywords = "Organic solvent, Organic-inorganic hybrid silicate, Protease, Sol-gel method, Transesterification",

author = "Furukawa, {Shin Ya} and Tsutomu Ono and Hiroyuki Ijima and Koei Kawakami",

year = "2002",

doi = "10.1023/A:1013876313806",

language = "English",

volume = "24",

pages = "13--16",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "1",

}

TY - JOUR

T1 - Activation of protease by sol-gel entrapment into organically modified hybrid silicates

AU - Furukawa, Shin Ya

AU - Ono, Tsutomu

AU - Ijima, Hiroyuki

AU - Kawakami, Koei

PY - 2002

Y1 - 2002

N2 - To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.

AB - To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.

KW - Organic solvent

KW - Organic-inorganic hybrid silicate

KW - Protease

KW - Sol-gel method

KW - Transesterification

UR - http://www.scopus.com/inward/record.url?scp=0036160284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036160284&partnerID=8YFLogxK

U2 - 10.1023/A:1013876313806

DO - 10.1023/A:1013876313806

M3 - Article

AN - SCOPUS:0036160284

SN - 0141-5492

VL - 24

SP - 13

EP - 16

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 1

ER -

Activation of protease by sol-gel entrapment into organically modified hybrid silicates

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this