Adenoviral targeting of malignant melanoma for fluorescenceguided surgery prevents recurrence in orthotopic nude-mouse models

Shuya Yano; Kiyoto Takehara; Hiroyuki Kishimoto; Yasuo Urata; Shunsuke Kagawa; Michael Bouvet; Toshiyoshi Fujiwara; Robert M. Hoffman

doi:10.18632/oncotarget.6670

Adenoviral targeting of malignant melanoma for fluorescenceguided surgery prevents recurrence in orthotopic nude-mouse models

Shuya Yano, Kiyoto Takehara, Hiroyuki Kishimoto, Yasuo Urata, Shunsuke Kagawa, Michael Bouvet, Toshiyoshi Fujiwara, Robert M. Hoffman

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in orthotopic mouse models. OBP-401-based fluorescence-guided surgery (FGS) resulted in the complete resection of malignant melanoma in the orthotopic models, where conventional bright-light surgery (BLS) could not. High-dose administration of OBP-401 enabled FGS without residual cancer cells or recurrence, due to its dual effect of cancer-cell labeling with GFP and killing.

Original language	English
Pages (from-to)	18558-18572
Number of pages	15
Journal	Oncotarget
Volume	7
Issue number	14
DOIs	https://doi.org/10.18632/oncotarget.6670
Publication status	Published - 2016

Keywords

Adenovirus
Fluorescence-guided surgery (FGS)
Melanoma
Nude mice
OBP-401

ASJC Scopus subject areas

Oncology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.6670

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title = "Adenoviral targeting of malignant melanoma for fluorescenceguided surgery prevents recurrence in orthotopic nude-mouse models",

abstract = "Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in orthotopic mouse models. OBP-401-based fluorescence-guided surgery (FGS) resulted in the complete resection of malignant melanoma in the orthotopic models, where conventional bright-light surgery (BLS) could not. High-dose administration of OBP-401 enabled FGS without residual cancer cells or recurrence, due to its dual effect of cancer-cell labeling with GFP and killing.",

keywords = "Adenovirus, Fluorescence-guided surgery (FGS), Melanoma, Nude mice, OBP-401",

author = "Shuya Yano and Kiyoto Takehara and Hiroyuki Kishimoto and Yasuo Urata and Shunsuke Kagawa and Michael Bouvet and Toshiyoshi Fujiwara and Hoffman, {Robert M.}",

note = "Funding Information: This study was supported by grants-in-Aid from the Ministry of Education, Science and culture, Japan and grants from the Ministry of Health and Welfare, Japan. This study was also supported in part by National Cancer Institute grants CA 132971 and CA142669.",

year = "2016",

doi = "10.18632/oncotarget.6670",

language = "English",

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T1 - Adenoviral targeting of malignant melanoma for fluorescenceguided surgery prevents recurrence in orthotopic nude-mouse models

AU - Yano, Shuya

AU - Takehara, Kiyoto

AU - Kishimoto, Hiroyuki

AU - Urata, Yasuo

AU - Kagawa, Shunsuke

AU - Bouvet, Michael

AU - Fujiwara, Toshiyoshi

AU - Hoffman, Robert M.

N1 - Funding Information: This study was supported by grants-in-Aid from the Ministry of Education, Science and culture, Japan and grants from the Ministry of Health and Welfare, Japan. This study was also supported in part by National Cancer Institute grants CA 132971 and CA142669.

PY - 2016

Y1 - 2016

N2 - Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in orthotopic mouse models. OBP-401-based fluorescence-guided surgery (FGS) resulted in the complete resection of malignant melanoma in the orthotopic models, where conventional bright-light surgery (BLS) could not. High-dose administration of OBP-401 enabled FGS without residual cancer cells or recurrence, due to its dual effect of cancer-cell labeling with GFP and killing.

AB - Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in orthotopic mouse models. OBP-401-based fluorescence-guided surgery (FGS) resulted in the complete resection of malignant melanoma in the orthotopic models, where conventional bright-light surgery (BLS) could not. High-dose administration of OBP-401 enabled FGS without residual cancer cells or recurrence, due to its dual effect of cancer-cell labeling with GFP and killing.

KW - Adenovirus

KW - Fluorescence-guided surgery (FGS)

KW - Melanoma

KW - Nude mice

KW - OBP-401

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Adenoviral targeting of malignant melanoma for fluorescenceguided surgery prevents recurrence in orthotopic nude-mouse models

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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