@article{13d6a3914edb4f6a869f3b0bf5d58a7c,
title = "Adipocyte-Specific Inhibition of Mir221/222 Ameliorates Diet-Induced Obesity Through Targeting Ddit4",
abstract = "MicroRNAs expressed in adipocytes are involved in transcriptional regulation of target mRNAs in obesity, but miRNAs critically involved in this process is not well characterized. Here, we identified upregulation of miR-221-3p and miR-222-3p in the white adipose tissues in C57BL/6 mice fed with high fat-high sucrose (HFHS) chow by RNA sequencing. Mir221 and Mir222 are paralogous genes and share the common seed sequence and Mir221/222AdipoKO mice fed with HFHS chow demonstrated resistance to the development of obesity compared with Mir221/222flox/y. Ddit4 is a direct target of Mir221 and Mir222, and the upregulation of Ddit4 in Mir221/222AdipoKO was associated with the suppression of TSC2 (tuberous sclerosis complex 2)/mammalian target of rapamycin complex 1 (mTORC1)/S6K (ribosomal protein S6 kinase) pathway. The overexpression of miR-222-3p linked to enhanced adipogenesis, and it may be a potential candidate for miRNA-based therapy.",
keywords = "Adipogenesis, adipose tissues, microRNA, mTORC1, non-coding RNAs",
author = "Satoshi Yamaguchi and Dongxiao Zhang and Akihiro Katayama and Naoko Kurooka and Ryosuke Sugawara and Albuayjan, {Haya Hamed Hassan} and Atsuko Nakatsuka and Jun Eguchi and Jun Wada",
note = "Funding Information: We acknowledge support from Central Research Laboratory, Okayama University Medical School; usage of Kubota High Speed Refrigerated Centrifuge Model 7780II, ABI PRISM3130, and producing paraffin blocks and sections. Funding Information: Conflict of Interest: JW receives speaker honoraria from Astra Zeneca, Daiichi Sankyo, Novartis, Novo Nordisk Pharma, Tanabe Mitsubishi and receives grant support from Astellas, Baxter, Bayer, Chugai, Dainippon Sumitomo, Kyowa Kirin, Novo Nordisk Pharma, Ono, Otsuka, Tanabe Mitsubishi, and Teijin. Funding Information: This work was supported by Grant-in-Aid for Young Scientists (19K17984), Grant-in-Aid for Scientific Research (B) (19H03675), Japan Agency for Medical Research and development (AMED, grant no: 17ek0210095h0001, 20ek0109445h0001). Publisher Copyright: Copyright {\textcopyright} 2022 Yamaguchi, Zhang, Katayama, Kurooka, Sugawara, Albuayjan, Nakatsuka, Eguchi and Wada.",
year = "2022",
month = jan,
day = "3",
doi = "10.3389/fendo.2021.750261",
language = "English",
volume = "12",
journal = "Frontiers in Endocrinology",
issn = "1664-2392",
publisher = "Frontiers Media S. A.",
}
