TY - JOUR
T1 - Adrenomedullin regulates the speed of oviductal fluid flow in cattle
AU - Yoshimoto, Yuka
AU - Nishie, Takumi
AU - Ito, Sayaka
AU - Kobayashi, Yoshihiko
AU - Yamamoto, Yuki
AU - Okuda, Kiyoshi
AU - Kimura, Koji
N1 - Funding Information:
We are grateful to Dr. T. Wakai (Okayama University, Okayama, Japan), Dr. R. Sakumoto, and Dr. K. Hayashi (National Agriculture and Food Research Organization, Ibaraki, Japan) for valuable technical supports. In addition, we thank the staff of the Assisted Reproductive Technology Center (Okayama University, Okayama, Japan) and the Department of Instrumental Analysis Advanced Science Research Center (Okayama University, Okayama, Japan) for their kind assistance. This study was supported by a Grant-in-Aid from the Morinaga Foundation for Health and Nutrition and partially supported by JSPS KAKENHI Grant Number JP17H05041.
Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2017/8
Y1 - 2017/8
N2 - Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM)—a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3 — promotes oviductal fluid flow in the ampulla of bovine oviducts. First, we examined the expression of ADM, CRLR, RAMP2, and RAMP3 mRNAs in isolated epithelial cells throughout the estrous cycle, and the localization of ADM receptor protein constituents in the ampulla. RAMP2 expression was significantly higher in the follicular phase. Furthermore, RAMP2 protein was detected only in ciliated cells, whereas CRLR and RAMP3 were detected in all epithelial cells. The effects of ADM and an ADM antagonist on fluid-flow speed were examined using microbeads in ampullary tissue. ADM antagonist decreased bead transport speed, and this decrease was reversed by ADM. In addition, ADM recovered the bead transport speed that decreased in the absence of calcium. Overall, our results suggest that ADM contributes to the regulation of oviductal fluid flow in ampulla.
AB - Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM)—a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3 — promotes oviductal fluid flow in the ampulla of bovine oviducts. First, we examined the expression of ADM, CRLR, RAMP2, and RAMP3 mRNAs in isolated epithelial cells throughout the estrous cycle, and the localization of ADM receptor protein constituents in the ampulla. RAMP2 expression was significantly higher in the follicular phase. Furthermore, RAMP2 protein was detected only in ciliated cells, whereas CRLR and RAMP3 were detected in all epithelial cells. The effects of ADM and an ADM antagonist on fluid-flow speed were examined using microbeads in ampullary tissue. ADM antagonist decreased bead transport speed, and this decrease was reversed by ADM. In addition, ADM recovered the bead transport speed that decreased in the absence of calcium. Overall, our results suggest that ADM contributes to the regulation of oviductal fluid flow in ampulla.
KW - RAMPs
KW - ciliary beating
KW - epithelial cell
KW - intracellular calcium
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U2 - 10.1002/mrd.22852
DO - 10.1002/mrd.22852
M3 - Article
C2 - 28621496
AN - SCOPUS:85026362083
SN - 1040-452X
VL - 84
SP - 712
EP - 718
JO - Gamete Research
JF - Gamete Research
IS - 8
ER -