TY - JOUR
T1 - Advances in induced Pluripotent Stem cell therapy for neurological diseases
AU - Yamashita, Toru
AU - Kawai, Hiromi
AU - Abe, Koji
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2011/11
Y1 - 2011/11
N2 - Induced Pluripotent Stem (iPS) cells are regarded to be potential supply source of cells for application to cell replacement therapies. Recent scientific papers indicated that human iPS cells are capable of differentiating into glutamatergic neurons, dopaminergic neurons, and motor neurons. However, tumorigenesis in iPS cells is a serious problem which should be overcome for clinical application. Recently, we evaluated the influence of ischemic condition on undifferentiated iPS cells within a transient Middle Cerebral Artery Occlusion (MCAO) using a mouse model. Undifferentiated iPS cells (5 × 105) were injected into the ipsilateral striatum and cortex, which were considered as the ischemic boundary zone at 24 hours after MCAO. Ischemic brains treated with iPS cells tended to form teratoma with larger volume compared with those in intact brains. c~Myc, Oct3/4 and Sox2 were strongly expressed in iPS-derived tumors of the ischemic brain. Above results indicated that the transcriptional factors integrated into the genome by retrovirus vectors might promote teratoma formation in the ischemic brain. Potential interaction between integrated transcriptional factors and iPS cell characteristics needs to be kept in mind, for the development of transplantation therapy.
AB - Induced Pluripotent Stem (iPS) cells are regarded to be potential supply source of cells for application to cell replacement therapies. Recent scientific papers indicated that human iPS cells are capable of differentiating into glutamatergic neurons, dopaminergic neurons, and motor neurons. However, tumorigenesis in iPS cells is a serious problem which should be overcome for clinical application. Recently, we evaluated the influence of ischemic condition on undifferentiated iPS cells within a transient Middle Cerebral Artery Occlusion (MCAO) using a mouse model. Undifferentiated iPS cells (5 × 105) were injected into the ipsilateral striatum and cortex, which were considered as the ischemic boundary zone at 24 hours after MCAO. Ischemic brains treated with iPS cells tended to form teratoma with larger volume compared with those in intact brains. c~Myc, Oct3/4 and Sox2 were strongly expressed in iPS-derived tumors of the ischemic brain. Above results indicated that the transcriptional factors integrated into the genome by retrovirus vectors might promote teratoma formation in the ischemic brain. Potential interaction between integrated transcriptional factors and iPS cell characteristics needs to be kept in mind, for the development of transplantation therapy.
KW - Cell transplantation
KW - Induced pluripotent stem cells
KW - Middle cerebral artery occlusion
KW - Tumorigenesis
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U2 - 10.7887/jcns.20.585
DO - 10.7887/jcns.20.585
M3 - Article
AN - SCOPUS:84055218251
SN - 0917-950X
VL - 20
SP - 585
EP - 588
JO - Japanese Journal of Neurosurgery
JF - Japanese Journal of Neurosurgery
IS - 8
ER -