Aging exacerbates restraint stress-induced inhibition of antigen-specific antibody production in mice

Yumiko Ichihara, Mitsuhiro Okano, Keiko Nishioka, Noriko Manabe, Naoto Ichihara, Fumihiko Jitsunari, Tazuko Fujiwara, Kazunori Nizhizaki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Background: We have recently found that exposure to acute restraint stress suppresses antigen-specific antibody production, including IgE, in a murine model of allergic rhinitis. Although age-related alterations in immune responses are known, it remains unclear whether aging modulates the antibody production under stressful conditions. In this study, we set out to determine the effects of aging on antibody production under acute restraint stress in mice. Methods: Both young and aged CBA/J mice were repeatedly sensitized intranasally with phospholipase A2 (PLA2) without adjuvants. Restraint stress was applied using uniform cylinders once a week for a continuous 8 h period, on 5 occasions in total. Blood samples were taken at 0, 20 and 30 days after primary sensitization, and production of PLA2-specific antibodies and levels of IL-4, IFN-γ, IL-10 and IL-1β in sera were determined by ELISA. Results: Repeated intranasal sensitization with PLA2 induced PLA2-specific IgE, IgG1 and IgG2a production in aged mice. We found that exposure to restraint stress significantly inhibited production of PLA2-specific IgE, IgG1 and IgG2a in aged mice. In addition, antibody production under restraint stress decreased significantly in aged mice when compared with young mice. No IL-4, IFN-γ, IL-10 or IL-1β were detected in sera from nonstressed or stressed aged mice. Conclusions: Aging exacerbates the immunosuppressive role of acute restraint stress in antigen-specific antibody production in mice.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalAllergology International
Issue number1
Publication statusPublished - 2009


  • Aged mouse
  • Immunosuppression
  • Phospholipase A2
  • Restraint stress
  • Specific antibody

ASJC Scopus subject areas

  • Immunology and Allergy


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