TY - JOUR
T1 - Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii
AU - Takahara, Hiroyuki
AU - Tsuji, Gento
AU - Kubo, Yasuyuki
AU - Yamamoto, Mikihiro
AU - Toyoda, Kazuhiro
AU - Inagaki, Yoshishige
AU - Ichinose, Yuki
AU - Shiraishi, Tomonori
N1 - Funding Information:
Acknowledgment This research was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (no. 15108001) from the Ministry of Education, Science, and Culture of Japan.
PY - 2004/4
Y1 - 2004/4
N2 - We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes.
AB - We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes.
KW - Agrobacterium tumefaciens
KW - Anthracnose
KW - Colletotrichum trifolii
KW - Random insertional mutagenesis
KW - T-DNA
KW - Transformation
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U2 - 10.1007/s10327-003-0099-y
DO - 10.1007/s10327-003-0099-y
M3 - Article
AN - SCOPUS:3042739655
SN - 1345-2630
VL - 70
SP - 93
EP - 96
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
IS - 2
ER -