TY - JOUR
T1 - Altered molecular attributes and antimicrobial resistance patterns of Vibrio cholerae O1 El Tor strains isolated from the cholera endemic regions of India
AU - Shaw, Sreeja
AU - Samanta, Prosenjit
AU - Chowdhury, Goutam
AU - Ghosh, Debjani
AU - Dey, Tanmoy Kumar
AU - Deb, Alok Kumar
AU - Ramamurthy, Thandavarayan
AU - Miyoshi, Shin ichi
AU - Ghosh, Amit
AU - Dutta, Shanta
AU - Mukhopadhyay, Asish Kumar
N1 - Funding Information:
This research was supported in part by the Indian Council of Medical Research (ICMR), Govt. of India; the National Institute of Infectious Diseases (NIID), Japan and the Japan Agency for Medical Research and Development (AMED) [grant number JP21wm0125004 (to SM)]. SS and DG acknowledge the CSIR fellowships [nos. 09/482(0071)/2019‐EMR‐I and 09/482(0074)/2019‐EMR‐I] received from the Council of Scientific and Industrial Research, India. GC acknowledges the ‘Scientist C’ position received from the Okayama University, Okayama, Japan. TKD acknowledges the fellowship received from the National Academy of Sciences, India. TR is the Indian National Science Academy (INSA) Senior Scientist. AG received JC Bose Chair Professorship of the National Academy of Sciences, India (no. NAS/2057/3/2015‐16, URL: http://www.nasi.org.in/ ). We are thankful to Mr. Mukul Roy for technical assistance in the PFGE experiment. We are grateful to the authority of different hospitals for providing strains used in this study. V. cholerae
Publisher Copyright:
© 2022 Society for Applied Microbiology.
PY - 2022
Y1 - 2022
N2 - Aims: The present study aimed to document the comparative analysis of differential hypervirulent features of Vibrio cholerae O1 strains isolated during 2018 from cholera endemic regions in Gujarat and Maharashtra (Western India) and West Bengal (Eastern India). Methods and Results: A total of 87 V. cholerae O1 clinical strains from Western India and 48 from Eastern India were analysed for a number of biotypic and genotypic features followed by antimicrobial resistance (AMR) profile. A novel polymerase chain reaction was designed to detect a large fragment deletion in the Vibrio seventh pandemic island II (VSP-II) genomic region, which is a significant genetic feature of the V. cholerae strains that have caused Yemen cholera outbreak. All the strains from Western India belong to the Ogawa serotype, polymyxin B-sensitive, hemolytic, had a deletion in VSP-II (VSP-IIC) region and carried Haitian genetic alleles of ctxB, tcpA and rtxA. Conversely, 14.6% (7/48) of the strains from Eastern India belonged to the Inaba serotype, polymyxin B-resistant, nonhemolytic, harboured VSP-II other than VSP-IIC type, classical ctxB, Haitian tcpA and El Tor rtxA alleles. Resistance to tetracycline and chloramphenicol has been observed in strains from both regions. Conclusions: This study showed hypervirulent, polymyxin B-sensitive epidemic causing strains in India along with the strains with polymyxin B-resistant and nonhemolytic traits that may spread and cause serious disease outcomes in future. Significance and impact of the study: The outcomes of this study can help to improve the understanding of the hyperpathogenic property of recently circulating pandemic Vibrio cholerae strains in India. Special attention is also needed for the monitoring of AMR surveillance because V. cholerae strains are losing susceptibility to many antibiotics used as a second line of defence in the treatment of cholera.
AB - Aims: The present study aimed to document the comparative analysis of differential hypervirulent features of Vibrio cholerae O1 strains isolated during 2018 from cholera endemic regions in Gujarat and Maharashtra (Western India) and West Bengal (Eastern India). Methods and Results: A total of 87 V. cholerae O1 clinical strains from Western India and 48 from Eastern India were analysed for a number of biotypic and genotypic features followed by antimicrobial resistance (AMR) profile. A novel polymerase chain reaction was designed to detect a large fragment deletion in the Vibrio seventh pandemic island II (VSP-II) genomic region, which is a significant genetic feature of the V. cholerae strains that have caused Yemen cholera outbreak. All the strains from Western India belong to the Ogawa serotype, polymyxin B-sensitive, hemolytic, had a deletion in VSP-II (VSP-IIC) region and carried Haitian genetic alleles of ctxB, tcpA and rtxA. Conversely, 14.6% (7/48) of the strains from Eastern India belonged to the Inaba serotype, polymyxin B-resistant, nonhemolytic, harboured VSP-II other than VSP-IIC type, classical ctxB, Haitian tcpA and El Tor rtxA alleles. Resistance to tetracycline and chloramphenicol has been observed in strains from both regions. Conclusions: This study showed hypervirulent, polymyxin B-sensitive epidemic causing strains in India along with the strains with polymyxin B-resistant and nonhemolytic traits that may spread and cause serious disease outcomes in future. Significance and impact of the study: The outcomes of this study can help to improve the understanding of the hyperpathogenic property of recently circulating pandemic Vibrio cholerae strains in India. Special attention is also needed for the monitoring of AMR surveillance because V. cholerae strains are losing susceptibility to many antibiotics used as a second line of defence in the treatment of cholera.
KW - cholera
KW - El Tor biotype
KW - nonhemolytic
KW - polymyxin B
KW - Vibrio cholerae
KW - vibrio seventh pandemic island II
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U2 - 10.1111/jam.15794
DO - 10.1111/jam.15794
M3 - Article
C2 - 36000378
AN - SCOPUS:85137492497
SN - 1364-5072
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
ER -
