Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension

Kazufumi Nakamura, Juichiro Shimizu, Noriyuki Kataoka, Katsushi Hashimoto, Tetsuya Ikeda, Hideki Fujio, Keiko Ohta-Ogo, Aiko Ogawa, Aya Miura, Satoshi Mohri, Satoshi Nagase, Hiroshi Morita, Kengo Fukushima Kusano, Hiroshi Date, Hiromi Matsubara, Seiichi Mochizuki, Ken Hashimoto, Fumihiko Kajiya, Tohru Ohe

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Background: Idiopathic pulmonary arterial hypertension (IPAH) is a disease characterized by progressively increased resistance of pulmonary arteries. In this study, we evaluated the mechanical property of single pulmonary artery smooth muscles cells (PASMC) from patients with IPAH and tested whether the PASMC showed abnormal response to a vasodilator by use of an atomic force microscope (AFM). Methods: PASMC were isolated and cultured from explanted lungs of 7 patients with IPAH (IPAH-PASMC). Normal vascular specimens from 3 patients with bronchogenic carcinoma were used as normal controls (normal PASMC). The nano/micro-order elasticity of five to ten living PASMC in each sample was measured by parabolic force curves of cantilever deflection/indentation obtained by using an AFM. The elasticity measurements were performed under control conditions and under condition of nitric oxide (NO) treatment (190 and 380 nmol/L). Results: There was no significant difference between nano/micro-order elasticity of normal PASMC and that of IPAH-PASMC under the control conditions. In normal PASMC, NO (190 and 380 nmol/L) significantly reduced (i.e., softened) the nano/micro-order elasticity. However, NO did not reduce elasticity in IPAH-PASMC, indicating higher vasodilator-resistive nano/micro-order rigidity in IPAH-PASMC. Conclusion: Nano/micro-order elasticity change in PASMC in response to vasodilation induced by NO is reduced in patients with IPAH.

Original languageEnglish
Pages (from-to)102-107
Number of pages6
JournalInternational Journal of Cardiology
Issue number1
Publication statusPublished - Apr 1 2010


  • Atomic force microscope
  • Nitric oxide
  • Pulmonary hypertension
  • Smooth muscle cell
  • Vasodilator

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


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