TY - CHAP
T1 - An analysis of pathological activities of CCN proteins in joint disorders
T2 - Mechanical stretch-mediated CCN2 expression in cultured meniscus cells
AU - Furumatsu, Takayuki
AU - Ozaki, Toshifumi
PY - 2017
Y1 - 2017
N2 - The multifunctional growth factor CYR61/CTGF/NOV (CCN) 2, also known as connective tissue growth factor, regulates cellular proliferation, differentiation, and tissue regeneration. Recent literatures have described important roles of CCN2 in the meniscus metabolism. However, the mechanical stressmediated transcriptional regulation of CCN2 in the meniscus remains unclear. The meniscus is a fibrocartilaginous tissue that controls complex biomechanics of the knee joint. Therefore, the injured unstable meniscus has a poor healing potential especially in the avascular inner region. In addition, dysfunction of the meniscus correlates with the progression of degenerative knee joint disorders and joint space narrowing. Here, we describe an experimental approach that investigates the distinct cellular behavior of inner and outer meniscus cells in response to mechanical stretch. Our experimental model can analyze the relationships between stretch-induced CCN2 expression and its functional role in the meniscus homeostasis.
AB - The multifunctional growth factor CYR61/CTGF/NOV (CCN) 2, also known as connective tissue growth factor, regulates cellular proliferation, differentiation, and tissue regeneration. Recent literatures have described important roles of CCN2 in the meniscus metabolism. However, the mechanical stressmediated transcriptional regulation of CCN2 in the meniscus remains unclear. The meniscus is a fibrocartilaginous tissue that controls complex biomechanics of the knee joint. Therefore, the injured unstable meniscus has a poor healing potential especially in the avascular inner region. In addition, dysfunction of the meniscus correlates with the progression of degenerative knee joint disorders and joint space narrowing. Here, we describe an experimental approach that investigates the distinct cellular behavior of inner and outer meniscus cells in response to mechanical stretch. Our experimental model can analyze the relationships between stretch-induced CCN2 expression and its functional role in the meniscus homeostasis.
KW - CCN2
KW - Mechanical stretch
KW - Meniscus
KW - Meniscus cells
KW - Transcriptional regulation
UR - http://www.scopus.com/inward/record.url?scp=84991716934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991716934&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6430-7_45
DO - 10.1007/978-1-4939-6430-7_45
M3 - Chapter
C2 - 27734404
AN - SCOPUS:84991716934
VL - 1489
T3 - Methods in Molecular Biology
SP - 533
EP - 542
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -