TY - JOUR
T1 - An in vitro screening method for DNA cytosine-C5-Methylase inhibitor
AU - Tamura, Takashi
AU - Kataoka, Atsushi
AU - Shu, Li Yin
AU - Ashida, Akihiko
AU - Tanaka, Hidehiko
AU - Inagaki, Kenji
N1 - Funding Information:
The present work was supported in part by a grant-in-aid for Scientific Research (07680688) from the Ministry of Education, Science and Culture of Japan to K.I.
PY - 2002
Y1 - 2002
N2 - A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and λ DNA as their substrate.
AB - A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and λ DNA as their substrate.
KW - DNA methylase
KW - S-Adenosyl-L-methionine
KW - Sinefungin
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U2 - 10.1080/1057563029001/4809
DO - 10.1080/1057563029001/4809
M3 - Article
C2 - 11942678
AN - SCOPUS:0036107952
SN - 1057-5634
VL - 16
SP - 25
EP - 27
JO - Natural Product Letters
JF - Natural Product Letters
IS - 1
ER -