TY - JOUR
T1 - An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 module is an essential early component of chitin-induced rice immunity
AU - Akamatsu, Akira
AU - Wong, Hann Lin
AU - Fujiwara, Masayuki
AU - Okuda, Jun
AU - Nishide, Keita
AU - Uno, Kazumi
AU - Imai, Keiko
AU - Umemura, Kenji
AU - Kawasaki, Tsutomu
AU - Kawano, Yoji
AU - Shimamoto, Ko
N1 - Funding Information:
We thank Dr. Naoto Shibuya for chitin and for insightful discussion. We appreciate Dr. Noriko Inada for technical advice on confocal laser microscopy and FRET analysis. We thank Dr. Yoichiro Fukao for mass spectrometry analysis. We are grateful to Professor Ian Smith for critical reading of the manuscript. We also thank Tomoko Aoi, Yukie Yoshioka, Yuko Tamaki, Junko Naritomi, and Yukiko Konomi for excellent technical assistance. A.A. was supported by a fellowship from the Japan Society for the Promotion of Science (JSPS). This research was supported by grants in aid from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics for Agricultural Innovation, PMI-0007) and the JSPS (13G0023) to K.S.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2013/4/17
Y1 - 2013/4/17
N2 - OsCEBiP, a chitin-binding protein, and OsCERK1, a receptor-like kinase, are plasma membrane (PM) proteins that form a receptor complex essential for fungal chitin-driven immune responses in rice. The signaling events immediately following chitin perception are unclear. Investigating the spatiotemporal regulation of the rice small GTPase OsRac1, we find that chitin induces rapid activation of OsRac1 at the PM. Searching for OsRac1 interactors, we identified OsRacGEF1 as a guanine nucleotide exchange factor for OsRac1. OsRacGEF1 interacts with OsCERK1 and is activated when its C-terminal S549 is phosphorylated by the cytoplasmic domain of OsCERK1 in response to chitin. Activated OsRacGEF1 is required for chitin-driven immune responses and resistance to rice blast fungus infection. Further, a protein complex including OsCERK1 and OsRacGEF1 is transported from the endoplasmic reticulum to the PM. Collectively, our results suggest that OsCEBiP, OsCERK1, OsRacGEF1, and OsRac1 function as key components of a "defensome" critically engaged early during chitin-induced immunity.
AB - OsCEBiP, a chitin-binding protein, and OsCERK1, a receptor-like kinase, are plasma membrane (PM) proteins that form a receptor complex essential for fungal chitin-driven immune responses in rice. The signaling events immediately following chitin perception are unclear. Investigating the spatiotemporal regulation of the rice small GTPase OsRac1, we find that chitin induces rapid activation of OsRac1 at the PM. Searching for OsRac1 interactors, we identified OsRacGEF1 as a guanine nucleotide exchange factor for OsRac1. OsRacGEF1 interacts with OsCERK1 and is activated when its C-terminal S549 is phosphorylated by the cytoplasmic domain of OsCERK1 in response to chitin. Activated OsRacGEF1 is required for chitin-driven immune responses and resistance to rice blast fungus infection. Further, a protein complex including OsCERK1 and OsRacGEF1 is transported from the endoplasmic reticulum to the PM. Collectively, our results suggest that OsCEBiP, OsCERK1, OsRacGEF1, and OsRac1 function as key components of a "defensome" critically engaged early during chitin-induced immunity.
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U2 - 10.1016/j.chom.2013.03.007
DO - 10.1016/j.chom.2013.03.007
M3 - Article
C2 - 23601108
AN - SCOPUS:84876391971
SN - 1931-3128
VL - 13
SP - 465
EP - 476
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 4
ER -